PROTEIN-CAPPED GOLD NANOPARTICLES OBTAINED BY  A GREEN SYNTHESIS METHOD

Cristina  COMAN; Loredana Florina  LEOPOLD; Olivia-Dumitriţa  RUGINĂ; Zoriţa  DIACONEASA; Pompei Florin  BOLFĂ; Nicolae  LEOPOLD; Maria  TOFANĂ; Carmen  SOCACIU

PROTEIN-CAPPED GOLD NANOPARTICLES OBTAINED BY A GREEN SYNTHESIS METHOD

Authors

Cristina COMAN Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Email: cristina.coman@usamvcluj.ro. https://orcid.org/0000-0002-7617-3763
Loredana Florina LEOPOLD Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Email: loredana.leopold@usamvcluj.ro. https://orcid.org/0000-0002-8084-1998
Olivia-Dumitriţa RUGINĂ Department of Chemistry and Biochemistry, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Email: dumitrita.rugina@gmail.com. https://orcid.org/0000-0003-4881-3273
Zoriţa DIACONEASA Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Email: zorita.sconta@usamvcluj.ro. https://orcid.org/0000-0002-5325-3859
Pompei Florin BOLFĂ Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Corresponding author: csocaciu@usamvcluj.ro. https://orcid.org/0000-0002-2903-1535
Nicolae LEOPOLD Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca, Romania. Corresponding author: csocaciu@usamvcluj.ro. https://orcid.org/0000-0002-2174-8064
Maria TOFANĂ Department of Food Science, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Email: maria.tofana@usamvcluj.ro. https://orcid.org/0000-0002-5872-7216
Carmen SOCACIU University of Agricultural Sciences and Veterinary Medicine; Research Center for Applied Biotechnolgy in Diagnosis and Molecular Therapy, BIODIATECH, Cluj-Napoca, Romania. Email: carmen.socaciu@usamvcluj.ro. https://orcid.org/0000-0002-7352-5057

Keywords:

gold nanoparticles, FTIR, TEM, HFL-1 cells

Abstract

A green synthesis protocol for the synthesis of colloidal gold nanoparticles is proposed. The synthesis involves the use of garlic (Allium sativum) extract as reducing agent. The reduction is carried out by slow addition of the Allium sativum extract to the chloroauric acid at boiling and gives spherically shaped nanoparticles, with 10-20 nm size distribution and mean diameters of 15 nm. The gold nanoparticles are characterized by UV-VIS spectroscopy, Fourier Transform Infrared Spectroscopy, Transmission Electron Microscopy, and Surface-Enhanced Raman Scattering. In vitro tests are also performed on human fetal lung fibroblast HFL-1 cells and the nanoparticles are found to be non-cytotoxic for the cells in the concentration range used in our study. Confocal microscopy shows that the particles are localised in the cell cytoplasm.

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STUDIA UBB CHEMIA, Volume 60 (LX), No. 1, March 2015

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Published

2015-03-30

How to Cite

COMAN, C. ., LEOPOLD, L. F. ., RUGINĂ, O.-D. ., DIACONEASA, Z. ., BOLFĂ, P. F. ., LEOPOLD, N. ., … SOCACIU, C. . (2015). PROTEIN-CAPPED GOLD NANOPARTICLES OBTAINED BY A GREEN SYNTHESIS METHOD. Studia Universitatis Babeș-Bolyai Chemia, 60(1), 119–126. Retrieved from https://studia.reviste.ubbcluj.ro/index.php/chemia/article/view/8409