A NOVEL PHENYLALANINE AMMONIA-LYASE FROM KANGIELLA KOREENSIS

Authors

  • Andrea VARGA Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Corresponding author: cslbencze@chem.ubbcluj.ro. https://orcid.org/0000-0002-4290-9226
  • Zsófia BATA Department of Organic Chemistry and Technology, Budapest University of Technology and Economics; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary. Email: bata.zsofia@mail.bme.hu. https://orcid.org/0000-0002-4840-9433
  • Pál CSUKA Department of Organic Chemistry and Technology, Budapest University of Technology and Economics; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary. Email: csuka.pal@vbk.bme.hu. https://orcid.org/0000-0003-3817-376X
  • Diana Monica BORDEA Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Email: dia.bordea@gmail.com.
  • Beáta G. VÉRTESSY Department of Biotechnology and Food Sciences, Budapest University of Technology and Economics; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary. Email: vertessy.beata@ttk.mta.hu. https://orcid.org/0000-0002-1288-2982
  • Adriana MARCOVICI Department of Clinical Pharmacology and Pharmacokinetics, Terapia SA – a Sun Pharma Company, Cluj-Napoca, Romania. Corresponding author: cslbencze@chem.ubbcluj.ro.
  • Florin-Dan IRIMIE Biocatalysis and Biotransformations Research Center, Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, Cluj-Napoca, Romania. Email: irimie@chem.ubbcluj.ro. https://orcid.org/0000-0003-2093-0136
  • László POPPE Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Hungary. Email: poppe.laszlo@vbk.bme.hu. https://orcid.org/0000-0002-8358-1378
  • László Csaba BENCZE Biocatalysis and Biotransformations Research Center, Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, Cluj-Napoca, Romania. Email: cslbencze@chem.ubbcluj.ro. https://orcid.org/0000-0003-0956-9749

DOI:

https://doi.org/10.24193/subbchem.2017.3.25

Keywords:

phenylalanine ammonia-lyase, Kangiella koreensis, protein expression, optimization

Abstract

This study describes the cloning of the gene encoding a novel phenylalanine ammonia-lyase from Kangiella koreensis (KkPAL) into pET19b expression vector. Optimization of protein expression and purification conditions yielded 15 mg pure soluble protein from one liter of E. coli culture. Enzymatic activity measurements of the ammonia elimination reaction from different natural aromatic amino acids proved the protein to be a phenylalanine ammonia-lyase. The isolated protein showed remarkably high, 81.7 °C melting temperature, making it especially suitable for biocatalytic applications.

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STUDIA UBB CHEMIA, Volume 62 (LXII), No. 3, September 2017

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Published

2017-09-29

How to Cite

VARGA, A. ., BATA, Z. ., CSUKA, P. ., BORDEA, D. M. ., VÉRTESSY, B. G. ., MARCOVICI, A. ., … BENCZE, L. C. . (2017). A NOVEL PHENYLALANINE AMMONIA-LYASE FROM KANGIELLA KOREENSIS. Studia Universitatis Babeș-Bolyai Chemia, 62(3), 293–308. https://doi.org/10.24193/subbchem.2017.3.25

Issue

Volume 62, No. 3, 2017

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