CONTINUOUS-FLOW ENZYMATIC KINETIC RESOLUTION MEDIATED BY A LIPASE NANOBIOCONJUGATE

Mădălina Elena  MOISĂ; László Csaba  BENCZE; Csaba  PAIZS; Monica Ioana  TOȘA

doi:10.24193/subbchem.2019.2.07

CONTINUOUS-FLOW ENZYMATIC KINETIC RESOLUTION MEDIATED BY A LIPASE NANOBIOCONJUGATE

Authors

Mădălina Elena MOISĂ Biocatalysis and Biotransformations Research Center, Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, Cluj-Napoca, Romania. Email: madalina.moisa@ubbcluj.ro. https://orcid.org/0000-0003-3215-5210
László Csaba BENCZE Biocatalysis and Biotransformations Research Center, Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, Cluj-Napoca, Romania. Email: cslbencze@chem.ubbcluj.ro. https://orcid.org/0000-0003-0956-9749
Csaba PAIZS Biocatalysis and Biotransformations Research Center, Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, Cluj-Napoca, Romania. Email: csaba.paizs@ubbcluj.ro. https://orcid.org/0000-0002-7403-7098
Monica Ioana TOȘA Biocatalysis and Biotransformations Research Center, Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, Cluj-Napoca, Romania. Email: mtosa@chem.ubbcluj.ro. https://orcid.org/0000-0003-3240-706X

DOI:

https://doi.org/10.24193/subbchem.2019.2.07

Keywords:

enzymatic kinetic resolution, secondary alcohol, lipase, continuous-flow process, batch process, productivity

Abstract

This study describes the development of a continuous-flow procedure for the enzymatic kinetic resolution of rac-1-phenylethan-1-ol with the clickable acylating agent 2,2,2-trifluoroethyl 2-(prop-2-yn-1-yl-oxy)acetate, which allows facile click reaction-based downstream, enabling the large-scale chemo-enzymatic synthesis of both secondary alcohol stereoisomers with high enantiopurity. The influence of flow rate, acylating agent and substrate concentration upon the productivity of the packed-bed reactor was investigated. In addition to this, the performances of continuous-flow and batch reactors were compared.

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STUDIA UBB CHEMIA, Volume 64 (LXIV), No.2, Tome I, June 2019

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Published

2019-06-03

How to Cite

MOISĂ, M. E. ., BENCZE, L. C. ., PAIZS, C. ., & TOȘA, M. I. . (2019). CONTINUOUS-FLOW ENZYMATIC KINETIC RESOLUTION MEDIATED BY A LIPASE NANOBIOCONJUGATE. Studia Universitatis Babeș-Bolyai Chemia, 64(2), 79–86. https://doi.org/10.24193/subbchem.2019.2.07