A NOVEL PHENYLALANINE AMMONIA-LYASE FROM KANGIELLA KOREENSIS

Authors

  • Andrea VARGA Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Corresponding author: cslbencze@chem.ubbcluj.ro. https://orcid.org/0000-0002-4290-9226
  • Zsófia BATA Department of Organic Chemistry and Technology, Budapest University of Technology and Economics; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary. Email: bata.zsofia@mail.bme.hu. https://orcid.org/0000-0002-4840-9433
  • Pál CSUKA Department of Organic Chemistry and Technology, Budapest University of Technology and Economics; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary. Email: csuka.pal@vbk.bme.hu. https://orcid.org/0000-0003-3817-376X
  • Diana Monica BORDEA Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Email: dia.bordea@gmail.com.
  • Beáta G. VÉRTESSY Department of Biotechnology and Food Sciences, Budapest University of Technology and Economics; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary. Email: vertessy.beata@ttk.mta.hu. https://orcid.org/0000-0002-1288-2982
  • Adriana MARCOVICI Department of Clinical Pharmacology and Pharmacokinetics, Terapia SA – a Sun Pharma Company, Cluj-Napoca, Romania. Corresponding author: cslbencze@chem.ubbcluj.ro.
  • Florin-Dan IRIMIE Biocatalysis and Biotransformations Research Center, Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, Cluj-Napoca, Romania. Email: irimie@chem.ubbcluj.ro. https://orcid.org/0000-0003-2093-0136
  • László POPPE Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Hungary. Email: poppe.laszlo@vbk.bme.hu. https://orcid.org/0000-0002-8358-1378
  • László Csaba BENCZE Biocatalysis and Biotransformations Research Center, Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, Cluj-Napoca, Romania. Email: cslbencze@chem.ubbcluj.ro. https://orcid.org/0000-0003-0956-9749

DOI:

https://doi.org/10.24193/subbchem.2017.3.25

Keywords:

phenylalanine ammonia-lyase, Kangiella koreensis, protein expression, optimization

Abstract

This study describes the cloning of the gene encoding a novel phenylalanine ammonia-lyase from Kangiella koreensis (KkPAL) into pET19b expression vector. Optimization of protein expression and purification conditions yielded 15 mg pure soluble protein from one liter of E. coli culture. Enzymatic activity measurements of the ammonia elimination reaction from different natural aromatic amino acids proved the protein to be a phenylalanine ammonia-lyase. The isolated protein showed remarkably high, 81.7 °C melting temperature, making it especially suitable for biocatalytic applications.

References

B. M. Nestl, S. C. Hammer, B. A. Nebel, B. Hauer, Angewandte Chemie, International Edition 2014, 53, 3070–3095.

L. Barredo, Microbial Enzymes and Biotransformations. 2005, Humana Press, Totowa.

D. S. Hodgins, Journal of Biological Chemistry 1971, 246, 2977–2985.

M. Petersen, J. Hans, U. Matern in Annual Plant Reviews, 2nd ed., Vol. 40, Biochemistry of Plant Secondary Metabolism (Ed.: M. Wink), Wiley-Blackwell, 2010, 182–257.

L. Feng, U. Wanninayake, S. Strom, J. Geiger, K. D. Walker, Biochemistry 2011, 50, 2919–2930.

S. D. Christenson, W. Liu, M. D. Toney, B. Shen, Journal of the American Chemical Society 2003, 125, 6062-6063; C. V. Christianson, T. J. Montavon, S. G. Van Lanen, B. Shen, S. D. Bruner, Biochemistry 2007, 46, 7205–7214.

J. A. Kyndt, T. E. Meyer, M. A. Cusanovich, J. J. Van Beeumen, FEBS Letters 2002, 512, 240–244.

L. Givot, T. A. Smith, R. H. Abeles, Journal of Biological Chemistry 1969, 244, 6341–6353; b) R. B. Wickner, Journal of Biological Chemistry 1969, 244, 6550–6552.

T. F. Schwede, J. Rétey, G. E. Schulz, Biochemistry 1999, 38, 5355–5361.

L. Poppe, Cs. Paizs, K. Kovács, F.D. Irimie, B.G. Vértessy in Methods in Molecular Biology, Vol. 794 (Eds.: L. Pollegioni, S. Servi), 2012, 3–19. Humana Press, Totowa.

F.B.J. van Assema, N. Sereinig (DSM0). WO 2008/031578, 2008 and PCT/EP 2007/007945, 2007.

D. Weiser, L.C. Bencze, G. Bánóczi, F. Ender, R. Kiss, E. Kókai, A. Szilágyi, B.G. Vértessy, O. Farkas, C. Paizs, L. Poppe, ChemBioChem, 2015, 16 , 2257–2402; b.) J. H. Bartha-Vári, M. I. Tosa, F.D. Irimie, D. Weiser, Z. Boros, B. G. Vértessy, Cs. Paizs, L. Poppe, ChemCatChem 2015, 7, 1122–1128; c.) F. Ender, D. Weiser, B. Nagy, L.C. Bencze, C. Paizs, P. Pálovics, L. Poppe, Journal of Flow Chemistry 2016 , 6, 43–52.

C. Han, J. Sikorski, A. Lapidus, M. Nolan, T. Glavina Del Rio, H. Tice,J. F. Cheng, S. Lucas, F. Chen, A. Copeland, N. Ivanova, K. Mavromatis, G. Ovchinnikova, A. Pati, D. Bruce, L. Goodwin, S. Pitluck, A. Chen, K. Palaniappan, M. Land, L. Hauser, Y. J. Chang, C. D. Jeffries, P. Chain, E. Saunders, T. Brettin, M. Göker, B. J. Tindall, J. Bristow, J. A. Eisen, V. Markowitz, P. Hugenholtz, N. C. Kyrpides, H. P. Klenk, J. C. Detter, Standards in Genomic Sciences, 2009, 1, 226–233.

Söding J, Bioinformatics, 2005, 21, 951–960.

S. Hou, J. H. Saw, K. S. Lee, T. A. Freitas, C. Belisle, Y. Kawarabayasi, S. P. Donachie, A. Pikina, M. Y. Galperin, E. V. Koonin, K.S. Makarova, M. V. Omelchenko, A. Sorokin, Y. I. Wolf, Q. X. Li, Y. S. Keum, S. Campbell, J. Denery, S. Aizawa, S. Shibata, A. Malahoff, M. Alam, Proceedings of the National Academy of Sciences USA, 2004, 101, 18036–18041.

G. V. Louie, M.E. Bowman, M. C. Moffitt, T. J. Baiga, B. S. Moore, J. P. Noel, Chemistry & Biology 2006, 13, 1327–1338.

F. Baneyx, Current Opinion in Biotechnology, 1999, 10, 411–421.

J. Sambrook, D. W. Russell, Molecular Cloning a Laboratory Manual. 3rd Edition, New York: Cold Spring Harbor Laboratory Press; 2001.

H. M. Sadeghi, M. Rabbani, E. Rismani, F. Moazen, F. Khodabakhsh, K. Dormiani, Y. Khazaei, Research in the Pharmaceutical Science, 2011, 6, 87–92.

R. Y. Li, C. Y. Cheng. Journal Bioscience and Bioengineering, 2009, 107, 512–515.

A. Vera, N. Gonzalez-Montalban, A. Aris, A. Villaverde, Biotechnology and Bioengineering, 2007, 96, 1101–1106.

J. A. Vasina, F. Baneyx, Protein Expression and Purification 1997, 9, 211–218.

J. Crowe, H. Dobeli, R. Gentz, E. Hochuli, D. Stiber, K. Hence, Methods in Molecular Biology, 1994, 31, 371–387.

T. Tanaka, M. Kubota, K. Samizo, Y. Nakajima, M. Hoshino, T. Kohno, E. Wakamatsu, Protein Expression and Purification, 1999, 1, 207–212.

A. Varga, G. Bánóczi, B. Nagy, L. C. Bencze, M. I. Toșa, Á. Gellért, F. D. Irimie, J. Rétey, L. Poppe C. Paizs, RSC Advances, 2016, 6, 56412–56420.

L. C. Bencze, A. Filip, G. Bánóczi, M. I. Toșa, F. D. Irimie, Á. Gellért, L. Poppe, C. Paizs, Organic & Biomolecular Chemistry, 2017, 15, 3717–3727.

STUDIA UBB CHEMIA, Volume 62 (LXII), No. 3, September 2017

Downloads

Published

2017-09-29

How to Cite

VARGA, A. ., BATA, Z. ., CSUKA, P. ., BORDEA, D. M. ., VÉRTESSY, B. G. ., MARCOVICI, A. ., … BENCZE, L. C. . (2017). A NOVEL PHENYLALANINE AMMONIA-LYASE FROM KANGIELLA KOREENSIS. Studia Universitatis Babeș-Bolyai Chemia, 62(3), 293–308. https://doi.org/10.24193/subbchem.2017.3.25

Issue

Volume 62, No. 3, 2017

Section

Articles

License

Copyright (c) 2017 Studia Universitatis Babeș-Bolyai Chemia

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Most read articles by the same author(s)

<< < 1 2 

Similar Articles

1 2 3 4 5 6 7 8 > >> 

You may also start an advanced similarity search for this article.