Effects of Some Turkish Plant Extracts on Carbonic Anhydrase and Cholinesterase Enzymes

İbrahim Seyda URAS; Murat KÜRŞAT; Belma KONUKLUGIL; Murat ŞENTÜRK

doi:10.24193/subbchem.2024.4.08

Authors

İbrahim Seyda URAS Department of Pharmacognosy, Faculty of Pharmacy, Agri Ibrahim Cecen University, Ağrı, Türkiye. Corresponding author: isuras@agri.edu.tr https://orcid.org/0000-0001-5945-8743
Murat KÜRŞAT Department of Biology, Faculty of Science and Arts, Bitlis Eren University, Bitlis, Türkiye. Email: mkursat@beu.edu.tr https://orcid.org/0000-0002-0861-4213
Belma KONUKLUGIL Department of Pharmacognosy, Faculty of Pharmacy, Lokman Hekim University, Ankara, Türkiye. Email: belma.konuklugil@lokmanhekim.edu.tr https://orcid.org/0000-0002-4753-0450
Murat ŞENTÜRK Department of Biochemistry, Faculty of Pharmacy, Agri Ibrahim Cecen University, Ağrı, Türkiye. Email: msenturk@agri.edu.tr https://orcid.org/0000-0001-5968-7511

DOI:

https://doi.org/10.24193/subbchem.2024.4.08

Keywords:

enzyme inhibition, Turkey flora, Zosima absinthifolia

Abstract

Cholinesterase inhibitors are valuable compounds that can be used in many different therapeutic applications, including Alzheimer’s disease. Carbonic anhydrase (CA) inhibitors constitute a pharmacological intervention employed for the management and alleviation of various medical conditions, including glaucoma, idiopathic intracranial hypertension. Turkey has a large and diverse flora, home to thousands of plant species. Hundreds of compounds of medicinal importance have been identified from the many plants in this flora. In this study, the inhibitory properties of seven different plant extracts on CA I and II, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were investigated. The tested extracts showed AChE inhibitory activity with values ranging from 1.26 to 4.20 μg/mL, BChE inhibitory activity with values ranging from 1.32 to 4.24 μg/mL, CA I inhibitory activity with values ranging from 0.74 to 1.82 μg/mL and CA II inhibitory activity with values ranging from 0.033 to 0.067 μg/mL. The extract of Zosima absinthifolia showed a very active inhibition profile against both AChE and BChE (IC₅₀1.26 ± 0.01 μg/mL for AChE and 1.32 ± 0.02 μg/mL for BChE). The results indicate that these extracts are potent cholinesterases inhibitors and specifically Zosima absinthifolia extract could be evaluated for further studies.

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