A NEW HPLC METHOD APPROACH FOR THE QUANTIFICATION OF QUERCETIN IN SEVEN DIFFERENT ANATOLIAN PLANT EXTRACTS

Abstract

The use of natural antioxidants, especially phenolic compounds derived from foods and plants, has been extensively investigated in the context of preventive and therapeutic medicine. Quercetin, a flavonoid of the flavonol class, exhibits multiple health-promoting properties, including antioxidant, antimicrobial, and antifungal activities. The present study aimed to quantify quercetin in seven plant species native to Türkiye: Zosima absinthifolia, Anarrhinum orientale, Fumaria asepala, Ferulago stellata, Salvia pseudeuphratica, Rhabdosciadium microcalycinum, and Diplotaenia cachrydifolia. A novel reversed-phase HPLC-DAD method was developed for the determination of quercetin in these plant extracts and subsequently validated in accordance with ICH guidelines. The method demonstrated linearity over the concentration range of 0.4–1.2 µg/mL (r² > 0.999), with mean recovery values ranging from 96.5% to 98.3%. All validation parameters were evaluated and confirmed in accordance with ICH guidelines, demonstrating the method's reliability for quantifying quercetin in plant matrices.