SCREENING OF POLYPHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF TANACETUM VULGARE FROM TRANSYLVANIA
Keywords:
Tanacetum vulgare, HPLC-MS, polyphenols, antioxidant, antimicrobial activitiesAbstract
The purpose of this study was to investigate the phenolic compounds and to evaluate the antioxidant and antimicrobial properties of Tanacetum vulgare flowers from different areas of Transylvania (Romania). The identification and quantification of major phenolic compounds were performed by a LC-MS method. The total polyphenols, caffeic acid derivatives and flavonoids content was spectrophotometrically determined. The antioxidant activity was evaluated using the DPPH bleaching method. The antimicrobial tests were performed using the disk diffusion assay. The phenolic profile showed the presence of phenolic acid derivatives (gentisic, caffeic, chlorogenic, p-coumaric, and ferulic acids), flavonoid glycosides (hyperoside, isoquercitrin, rutin and quercitrin) and flavonoid aglycones (quercetin, patuletin, luteolin, kaempferol, apigenin). The extracts of T. vulgare from Sibiu contain higher amounts of total polyphenols, flavonoids and caffeic acid derivatives than the extracts of T. vulgare from Alba. Both ethanolic extracts showed good antioxidant activity and low inhibitory activity against Gram-positive bacteria. The displayed potent antioxidant activity of these extracts supports the ethnomedicinal uses for this species.
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