LC-MS/MS METHOD FOR DETERMINATION OF L-Α-PHOSPHATIDYLCHOLINE FROM SOYBEAN

Dorina  SIMEDRU; Anca  NAGHIU; Oana  CADAR; Marius Lucian  DORDAI; Vlad-Emil  LUCA; Ioan  SIMON

LC-MS/MS METHOD FOR DETERMINATION OF L-Α-PHOSPHATIDYLCHOLINE FROM SOYBEAN

Authors

Dorina SIMEDRU Research Institute for Analytical Instrumentation (INCDO-INOE 2000), Cluj-Napoca, Romania. Email: dorina.simedru@icia.ro. https://orcid.org/0000-0003-4914-6986
Anca NAGHIU Research Institute for Analytical Instrumentation (INCDO-INOE 2000), Cluj-Napoca, Romania. Email: ancanaghiu@gmail.com. https://orcid.org/0000-0003-1418-689X
Oana CADAR Research Institute for Analytical Instrumentation (INCDO-INOE 2000), Cluj-Napoca, Romania. Email: oana.cadar@icia.ro. https://orcid.org/0000-0002-0879-9211
Marius Lucian DORDAI University of Agricultural Sciences and Veterinary Medicine (USAMV), Cluj-Napoca, Romania. Email: lucian.dordai@icia.ro. https://orcid.org/0000-0001-5255-4003
Vlad-Emil LUCA Department of Surgery, University of Agricultural Sciences and Veterinary Medicine (USAMV), Cluj-Napoca, Romania. Email: eluca@usamvcluj.ro. https://orcid.org/0000-0002-8683-6225
Ioan SIMON “Iuliu Hatieganu” University of Medicine and Pharmacy (UMF), Cluj-Napoca, Cluj-Napoca, Romania. Email: isimon@umfcluj.ro. https://orcid.org/0000-0002-0874-9089

Keywords:

lecithin, α-phosphatidylcholine, soybean, solid-liquid extraction, detection limits, accuracy

Abstract

The purpose of this study was to develop a quick procedure for the determination of α-phosphatidylcholine from soybean. The procedure is based on a simple solid-liquid extraction followed by a quick analytical method carried out with an LC-ESI-MS/MS system. A chromatographic column of 2×50 mm with a particle size of 2.5 µm was used. The LC-ESI-MS/MS method was developed in positive ionization mode. The analytical method was described in terms of linearity, detection and quantification limits, accuracy and precision and matrix effect. The calibration curve was developed in the range of 5 to 200 ng/ml with a correlation coefficient r² of 0.995 and detection limit of 0.5 ng/ml. The extraction method was tested for the recovery degree. The recovery obtained was 97.2±1.2%. The method was used to determine the content of α-phosphatidylcholine from five soybean varieties from Romanian market. No significant differences were obtained between the five varieties regarding the α-phosphatidylcholine content.

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STUDIA UBB CHEMIA, Volume 61 (LXI), No. 3, September 2016

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Published

2016-09-30

How to Cite

SIMEDRU, D. ., NAGHIU, A. ., CADAR, O. ., DORDAI, M. L. ., LUCA, V.-E. ., & SIMON, I. . (2016). LC-MS/MS METHOD FOR DETERMINATION OF L-Α-PHOSPHATIDYLCHOLINE FROM SOYBEAN. Studia Universitatis Babeș-Bolyai Chemia, 61(3), 407–414. Retrieved from https://studia.reviste.ubbcluj.ro/index.php/chemia/article/view/8363