THE PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT CAPACITY DETERMINATION OF FIVE HYPERICUM SPECIES

Authors

  • Adaeze Adejoke ADEHUWA-OLABODE University of Portsmouth, Faculty of Pharmacy, St. Michael's Building, White Swan Road, PO1 2DT, Portsmouth, United Kingdom.
  • Asmita SAUTREAU University of Portsmouth, Faculty of Pharmacy, St. Michael's Building, White Swan Road, PO1 2DT, Portsmouth, United Kingdom. https://orcid.org/0000-0002-0376-8643
  • Laurian VLASE Iuliu Hațieganu University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Technology and Biopharmacy, 8 Victor Babeș str., RO-400012, Cluj-Napoca, Romania. https://orcid.org/0000-0002-0664-3387
  • Ana-Maria VLASE Iuliu Hațieganu University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Botany, 23 Marinescu str., RO-400337, Cluj-Napoca, Romania. *Corresponding author: anamaria.gheldiu@yahoo.com https://orcid.org/0000-0003-4865-0777
  • Dana MUNTEAN Iuliu Hațieganu University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Technology and Biopharmacy, 8 Victor Babeș str., RO-400012, Cluj-Napoca, Romania. https://orcid.org/0000-0002-3914-0110

DOI:

https://doi.org/10.24193/subbchem.2022.3.02

Keywords:

Phytochemical analysis, antioxidant activity, Hypericum species, hyperforin, hypericin.

Abstract

Medicinal plants are a valuable source of lead compounds and novel drugs. The Hypericum L. species traditionally treats skin wounds, sciatica, and depression. Presently, only a small percentage of the Hypericum species have been phytochemically characterised and 60% still requires analysis. Ethanolic and methanolic extracts were obtained by ultrasound assisted extraction and maceration extraction methods. Polyphenols, sterols, methoxyflavones, hyperforin and hypericin were detected through HPLC-MS analysis. ABTS assay was used to evaluate the total antioxidant capacity.
H. maculatum and H. moserianum had the highest antioxidant capacity. The most abundant polyphenol in H. humifusum, H. moserianum and H. miracle-pistache was chlorogenic acid. For H. maculatum and H. perforatum, hyperoside and rutoside were the most abundant polyphenols. Sterols and methoxyflavones were quantified for the first time in all the species. β-sitosterol was the most abundant sterol across all species and ergosterol was absent in all species. Hispidulin was the only methoxyflavone (in small concentrations) found in all species except H. miracle-pistache. Hypericin was absent in H. moserianum and H. miracle-pistache and was most abundant in H. maculatum. Interestingly, H. miracle-pistache had higher concentrations of hyperforin than H. perforatum. The phytochemical profile of analysed Hypericum species prove to be a valuable bioactive’s source.

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Published

2022-09-30

How to Cite

ADEHUWA-OLABODE, A. A., SAUTREAU, A., VLASE, L., VLASE, A.-M., & MUNTEAN, D. (2022). THE PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT CAPACITY DETERMINATION OF FIVE HYPERICUM SPECIES. Studia Universitatis Babeș-Bolyai Chemia, 67(3), 17–35. https://doi.org/10.24193/subbchem.2022.3.02

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