HPLC ANALYSIS, ANTIMICROBIAL AND ANTIFUNGAL ACTIVITY OF AN EXPERIMENTAL PLANT BASED GEL, FOR ENDODONTIC USAGE

Authors

  • Andrada VOINA ȚONEA Department of Dental Matherials, Faculty of Dental Medicine, ‟Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania. Email: andrada.tonea@umfcluj.ro. https://orcid.org/0000-0003-4274-3016
  • Liviu Ioan OANA Department of Surgery, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Email: oanaliviu2008@yahoo.com. https://orcid.org/0000-0002-5624-6902
  • Mîndra Eugenia BADEA Department of Prevention in Dental Medicine, Iuliu Hațieganu University of Medicine and Farmacy, Cluj-Napoca, Romania. Email: mebadea@umfcluj.ro. https://orcid.org/0000-0003-3136-6956
  • Sorina SAVA Department of Prosthodontics and Dental Materials, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. Email: savasorina@yahoo.com. https://orcid.org/0000-0001-8658-1384
  • Corina VOINA Department of Odontology and Oral Pathology, Faculty of Dental Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania. Corresponding author: andrada.tonea@umfcluj.ro.
  • Florica RANGA Department of Agrifood Biochemistry, University of Agricultural Sciences and Veterinary Medicine (USAMV), Cluj-Napoca, Romania. Email: floricutza_ro@yahoo.com. https://orcid.org/0000-0002-4694-7932
  • Dan-Cristian VODNAR Department of Agrifood Biochemistry, University of Agricultural Sciences and Veterinary Medicine (USAMV), Cluj-Napoca, Romania. Email: dan.vodnar@usamvcluj.ro. https://orcid.org/0000-0001-5407-8071

Keywords:

antimicrobial, antifungal, HPLC/ESI( )-MS, agar-diffusion, microdilution, Arctium lappa, Aloe vera

Abstract

The aim of this study is to investigate the active compounds of an experimental gel and to evaluate its antimicrobial and antifungal effects. This gel consists of a mix extract of Arctium lappa L. root powder and Aloe vera (L.) Burm.f. gel and is used in endodontic procedures. With the help of HPLC/ESI(+)-MS method, various active compounds were identified in the experimental extract. For the evaluation of the antimicrobial and antifungal activity, microorganisms commonly found in endodontic infections were used. The agar-diffusion method was performed for each plant formula and the microdilution method was used to obtain the minimum inhibitory concentration and also the minimum bactericidal concentration. Clear inhibition zones around discs indicated the presence of antimicrobial and antifungal activity for the disc diffusion assay. The minimum inhibitory and bactericidal concentration obtained were compared to the antibiotics or antifungals, specific for each microorganism.

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Published

2016-12-30

How to Cite

VOINA ȚONEA, A. ., OANA, L. I. ., BADEA, M. E. ., SAVA, S. ., VOINA, C. ., RANGA, F. ., & VODNAR, D.-C. . (2016). HPLC ANALYSIS, ANTIMICROBIAL AND ANTIFUNGAL ACTIVITY OF AN EXPERIMENTAL PLANT BASED GEL, FOR ENDODONTIC USAGE. Studia Universitatis Babeș-Bolyai Chemia, 61(4), 53–68. Retrieved from https://studia.reviste.ubbcluj.ro/index.php/chemia/article/view/8379

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