HPLC ANALYSIS, ANTIMICROBIAL AND ANTIFUNGAL ACTIVITY OF AN EXPERIMENTAL PLANT BASED GEL, FOR ENDODONTIC USAGE

Andrada  VOINA ȚONEA; Liviu Ioan  OANA; Mîndra Eugenia  BADEA; Sorina  SAVA; Corina  VOINA; Florica  RANGA; Dan-Cristian  VODNAR

HPLC ANALYSIS, ANTIMICROBIAL AND ANTIFUNGAL ACTIVITY OF AN EXPERIMENTAL PLANT BASED GEL, FOR ENDODONTIC USAGE

Authors

Andrada VOINA ȚONEA Department of Dental Matherials, Faculty of Dental Medicine, ‟Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania. Email: andrada.tonea@umfcluj.ro. https://orcid.org/0000-0003-4274-3016
Liviu Ioan OANA Department of Surgery, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Email: oanaliviu2008@yahoo.com. https://orcid.org/0000-0002-5624-6902
Mîndra Eugenia BADEA Department of Prevention in Dental Medicine, Iuliu Hațieganu University of Medicine and Farmacy, Cluj-Napoca, Romania. Email: mebadea@umfcluj.ro. https://orcid.org/0000-0003-3136-6956
Sorina SAVA Department of Prosthodontics and Dental Materials, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. Email: savasorina@yahoo.com. https://orcid.org/0000-0001-8658-1384
Corina VOINA Department of Odontology and Oral Pathology, Faculty of Dental Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania. Corresponding author: andrada.tonea@umfcluj.ro.
Florica RANGA Department of Agrifood Biochemistry, University of Agricultural Sciences and Veterinary Medicine (USAMV), Cluj-Napoca, Romania. Email: floricutza_ro@yahoo.com. https://orcid.org/0000-0002-4694-7932
Dan-Cristian VODNAR Department of Agrifood Biochemistry, University of Agricultural Sciences and Veterinary Medicine (USAMV), Cluj-Napoca, Romania. Email: dan.vodnar@usamvcluj.ro. https://orcid.org/0000-0001-5407-8071

Keywords:

antimicrobial, antifungal, HPLC/ESI( )-MS, agar-diffusion, microdilution, Arctium lappa, Aloe vera

Abstract

The aim of this study is to investigate the active compounds of an experimental gel and to evaluate its antimicrobial and antifungal effects. This gel consists of a mix extract of Arctium lappa L. root powder and Aloe vera (L.) Burm.f. gel and is used in endodontic procedures. With the help of HPLC/ESI(+)-MS method, various active compounds were identified in the experimental extract. For the evaluation of the antimicrobial and antifungal activity, microorganisms commonly found in endodontic infections were used. The agar-diffusion method was performed for each plant formula and the microdilution method was used to obtain the minimum inhibitory concentration and also the minimum bactericidal concentration. Clear inhibition zones around discs indicated the presence of antimicrobial and antifungal activity for the disc diffusion assay. The minimum inhibitory and bactericidal concentration obtained were compared to the antibiotics or antifungals, specific for each microorganism.

References

Y.S. Chan, L.N. Cheng, J.H. Wu, E. Chan, Y.W. Kwan, S.M. Yuen Lee, G.P Heng Leung, P. Hoi-Fu Yu, S. W. Chan, Inflammopharmacology, 2011, 19, 245

S.E. Edwards, I. da Costa Rocha, E.M. Williamson, M. Heinrich, “Burdock, in Phytopharmacy: An Evidence-Based Guide to Herbal Medical Products”, John Wiley & Sons, Ltd, Chichester, 2015, chapter 17

A. Miglani, R. K. Manchanda, Homeopathy, 2014, 103, 203

T.G. Tutin, V.H. Heywood, N.A. Burges, D.M. Moore, D.H. Valentine, S.M. Walters, D.A. Webb, “Flora Europaea: Plantaginaceae to Compositae (and Rubiaceae)”, Cambridge University Press, Cambridge, 2010, 215

J. Liu, Y.Z. Cai, R.N.S. Wong, C. Kai-Fai Lee, S. Chi Wai Tang, S. Cho Wing Sze, Y. Tong, Y. Zhang, Journal of Agricultural and Food Chemistry, 2012, 60, 4067

P. Duh, Journal of the American Oil Chemists' Society, 1998, 75, 455

J. Cao, C. Li, P. Zhang, X. Cao, T. Huang, B. Yungui, C. Kaoshan, African Journal of Biotechnology, 2012, 11, 9079

F.S. Predes, A. Ruiz, J.E. Carvalho, M.A. Foglio, H. Dolder, BMC Complementary and Alternative Medicine, 2011, 11, 1

Z. Lou, H. Wang, W. Lv, C. Ma, Z. Wang, S. Chen, Food Control, 2010, 21, 1272

T.G. Tutin, V.H. Heywood, N.A. Burges, D.M. Moore, D.H. Valentine, S.M. Walters, D.A. Webb, “Flora Europaea: Alismataceae to Orchidceae (Monocotyledones)”, Cambridge University Press, Cambridge, 2005, 19

K. Eshun, Q He, Critical reviews in food science and nutrition, 2004, 44, 91

C.T. Ramachandra, P.S. Rao, American Journal of Agricultural and Biological Sciences, 2008, 3, 502

Jo. H. Hamman, Molecules 2008, 13, 1599

M. Saito, M. Tanaka, E. Misawa, R. Yao, K. Nabeshima, K. Yamauchi, F. Abe, Y. Yamamoto, F. Furukawa, Bioscience, Biotechnology, and Biochemistry, 2016, 80, 1416

S.E. Dal'Belo, L.R. Gaspar, M. Campos, Skin Research and Technology, 2006, 12, 241

J.A. Vinson, H.A. Kharrat, L. Andreoli, Phytomedicine, 2005, 12, 760

A. Hindustan, A. Chandrashekar, B. Anil Kumar, B. Reddy B.V. Ravindra, V.S Lakshmi, International Journal of PharmTech Research, 2010, 2, 1018

M.R. Akhoondinasab, M. Akhoondinasab, M. Saberi, World Journal of Plastic Surgery, 2014, 3, 29

M. Tarameshloo, M. Norouzian, S. Zarein-Dolab, M. Dadpay, J.Mohsenifar, R. Gazor, Anatomy and Cell Biology, 2012, 45, 170

M.R. Tabandeh, A. O’ryan, A. Mohammad Valipour, International Journal of Biological Macromolecules, 2014, 65, 424

L Cellini, S Di Bartolomeo, E Di Campli, S. Genovese, M. Locatelli, M Di Giulio, Letters in Applied Microbioogy,2014, 59, 43

R. Pandey, A Mishra, Applied Biochemistry and Biotechnology, 2010, 160, 1356

Bernardes, M. P.F. Rodrigues, G.K. Bacelli, E. Munin, L.P. Alves, M.S. Costa, Mycoses, 2012, 55, 257

V. Singh, S.P. Singh, M. Singh, A.K. Gupta, A. Kumar, Applied Biochemistry and Biotechnology, 2015, 175, 2542

M.R. Saini, P.K. Goyal, G. Chaudhary, Journal of Environmental Pathology, Toxicology and Oncology, 2010, 29, 127

W. Lowther, K. Lorick, S.D. Lawrence, W.S. Yeow, Transgenic Research, 2012, 21, 1349

J.R. de Oliveira, R.B.de Aguiar Almeida, P. das Graças Figueiredo Vilela, F.E. de Oliveira, R.F. da Rocha, A.O.C. Jorge, L.D. de Oliveira, Archives of Oral Biology, 2014, 59, 808

R. Pradeep, E. Agarwal, S.B. Naik, Journal of Periodontology, 2012; 83, 797

A. Ahmadi, Chinese Journal of Integrative Medicine, 2012, 18, 635

G. Bhat, P. Kudva, V. Dodwad, Journal of Indian Society of Periodontology, 2011, 15, 205

P. Chantarawaratit, P. Sangvanich, W. Banlunara, K. Soontornvipart, P. Thunyakitpisal, Journal of Periodontal Research, 2014, 49, 164

A. Gatot, J. Arbelle, A. Leiberman, I. Yanai-Inbar, Journal of Endodontics, 1991, 17, 573

R.S.Chhabra, J.E. Huff, J.K. Haseman, M.R. Elwell, A.C. Peters, Food and Chemical Toxicology, 1991, 29,119

B.P.F.A. Gomes, M.E. Vianna, A.A. Zaia, J.F.A. Almeida, F.J. Souza-Filho, C.C.C. Ferraz, Brazilian Dental Journal, 2013, 24, 89

B.B. Jyoti, Journal of Conservative Dentistry, 2005, 8, 31

J.V. Pereira, D.C. Baldoqui Bergamo, J.O. Pereira, S. de Castro França, R.C. Linhares Rodrigues Pietro, Y.T.C. Silva-Sousa, Brazilian Dental Journal, 2005, 16, 192

M. Pujar, S.D. Makandar, International Journal of Contemporary Dentistry, 2011, 2, 34

A. Abbaszadegan, S. Sahebi, A. Gholami, Journal of Investigative and Clinical Dentistry, 2016, 7, 93

O.R.Casian, M. Parvu, L. Vlase, M. Tamas, Fitoterapia, 2007, 78, 219

M.N. Clifford, Journal of the science of Food and Agriculture, 2000, 80, 1033

Farah, M. Monteiro, C.M. Donangelo, S. Lafay, The Journal of Nutrition, 2008, 138, 2309

R. Ferracane, G. Graziani, M. Gallo, V. Fogliano, A. Ritieni, Journal of Pharmaceutical and Biomedical Analysis, 2010, 51, 399

Z. Lou, H. Wang, S. Zhu, C. Ma, Z. Wang, Journal of Food Science, 2011, 76, M398

C.M. Ma, M. Kully, J.K. Khan, M. Hattori, M. Daneshtalab, Bioorganic & Medicinal Chemistry, 2007, 15, 6830

N. Venkateshbabu, S. Anand, M. Abarajithan, S.O. Sheriff, P.S. Jacob, N. Sonia, The Open Dentistry Journal, 2016, 10, 214

N. Lourith, M. Kanlayavattanakul, Journal of Oleo Science, 2013, 62, 235

J. Zhang, J. Chen, Z. Liang, C. Zhao, Chemistry & Biodiversity, 2014, 11, 1

Y. Cai, Q. Luo, M. Sun, H. Corke, Life Sciences, 2004, 74, 2157

S. Su, X. Cheng, M. Wink, Journal of Pharmacy and Pharmacology, 2015, 67, 1316

S. Su, X. Cheng, M. Wink, Phytomedicine, 2015, 22, 301

F. Zhao, L. Wang, K. Liu - Journal of Ethnopharmacology, 2009, 122, 457

S. Lee, S, Shin, H. Kim, S. Han, K. Kim, J. Kwon, J. Kwak, C. Lee, N. Ha, D. Yim, K. Kim, Journal of Inflammation, 2011, 8, 1

J.E. Kim, H.J. Kim, S. Pandit, K.W. Chang, J.G. Jeon, Fitoterapia, 2011, 82, 352

V. Cataldi, S. Di Bartolomeo, E. Di Campli, A. Nostro, L. Cellini, M. Di Giulio, International Journal of Immunopathology & Pharmacology, 2015, 28, 595

E. Cook, The Internet Journal of Microbiology, 2008; 4, 1

S. Alemdar, S. Agaoglu, Journal of Animal and Veterinary Advances, 2009, 8, 99

T. Hatano, M. Kusuda, K. Inada, T. Ogawa, S. Shiota, T. Tsuchiya, T. Yoshida, Phytochemistry, 2005, 66, 2047

S.K. Agarwal, S.S. Singh, S. Verma, S. Kumar, Journal of Ethnopharmacology, 2000, 72, 43

T. Pecere, M.V. Gazzola, C. Mucignat, C. Parolin, A. Cavaggioni, G. Basso, A. Diaspro, B. Salvato, M. Carli, G. Palu, Cancer Research, 2000, 60, 2800

H.Z. Lee, British Journal of Pharmacology, 2001, 134, 1093

P.L. Kuo, T.C. Lin, C.C. Lin, Life Sciences, 2002, 71, 1879

M.M. Menezes, M.C.Valera, A.O.C Jorge, C.Y.Coga-Ito, C.H.R Camargo, M.N.G Mancini, International Endodontic Journal, 2004, 37, 311

P. Sudbery, N. Gow, J. Berman, Trends in Microbiology, 2004, 12, 317

J.F. Siqueira Jr., B. H. Sen, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2004, 5, 632

M. Evans J.K. Davies, G. Sundqvist, D. Figdor, International Endodontic Journal, 2002, 35, 221

H.H. Hancock III, A. Sigurdsson, M. Trope, J. Moiseiwitsch, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2001, 91, 579

N. Vivacqua-Gomes, E.D. Gurgel-Filho, B.P.F.A. Gomes, C.C.R. Ferraz, A.A. Zaia, F.J. Souza-Filho, International Endodontic Journal, 2005, 38, 697

I.N. Rôças, J.F. Siqueira, Journal of Clinical Microbiology, 2012, 50, 1721

F.C.S. Chu, C.S.P. Tsang, T.W. Chow, L.P. Samaranayake, Journal of Endodontics, 2005, 31, 424

L.E.C. de Paz Villanueva, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2002, 93, 179

M.P. Riggio, A. Lennon, Journal of Medical Microbiology, 2002, 51, 1097

A. Gomez, D. A. Gerber, E. Zambrano, N. Banaei, Anaerobe, 2015, 36, 53

A.M.T. Öncül, E. Uzunoğlu, Z.C. Karahan, A.M. Aksoy, R. Kişnişci, Ö. Karaahmetoğlu, Journal of Oral and Maxillofacial Surgery, 2015, 73, 259.

STUDIA UBB CHEMIA, Volume 61 (LXI), No. 4, December 2016

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Published

2016-12-30

How to Cite

VOINA ȚONEA, A. ., OANA, L. I. ., BADEA, M. E. ., SAVA, S. ., VOINA, C. ., RANGA, F. ., & VODNAR, D.-C. . (2016). HPLC ANALYSIS, ANTIMICROBIAL AND ANTIFUNGAL ACTIVITY OF AN EXPERIMENTAL PLANT BASED GEL, FOR ENDODONTIC USAGE. Studia Universitatis Babeș-Bolyai Chemia, 61(4), 53–68. Retrieved from https://studia.reviste.ubbcluj.ro/index.php/chemia/article/view/8379