DETERMINATION OF ANDROSTERONE FROM CELERY BY A NEW VALIDATED LC-MS/MS METHOD

Dorina  SIMEDRU; Anca  NAGHIU; Oana  CADAR; Marius Lucian  DORDAI; Vlad-Emil  LUCA; Ioan  SIMON

DETERMINATION OF ANDROSTERONE FROM CELERY BY A NEW VALIDATED LC-MS/MS METHOD

Authors

Dorina SIMEDRU Research Institute for Analytical Instrumentation (INCDO-INOE 2000), Cluj-Napoca, Romania. Email: dorina.simedru@icia.ro. https://orcid.org/0000-0003-4914-6986
Anca NAGHIU Research Institute for Analytical Instrumentation (INCDO-INOE 2000), Cluj-Napoca, Romania. Email: ancanaghiu@gmail.com. https://orcid.org/0000-0003-1418-689X
Oana CADAR Research Institute for Analytical Instrumentation (INCDO-INOE 2000), Cluj-Napoca, Romania. Email: oana.cadar@icia.ro. https://orcid.org/0000-0002-0879-9211
Marius Lucian DORDAI University of Agricultural Sciences and Veterinary Medicine (USAMV), Cluj-Napoca, Romania. Email: lucian.dordai@icia.ro. https://orcid.org/0000-0001-5255-4003
Vlad-Emil LUCA Department of Surgery, University of Agricultural Sciences and Veterinary Medicine (USAMV), Cluj-Napoca, Romania. Email: eluca@usamvcluj.ro. https://orcid.org/0000-0002-8683-6225
Ioan SIMON “Iuliu Hatieganu” University of Medicine and Pharmacy (UMF), Cluj-Napoca, Cluj-Napoca, Romania. Email: isimon@umfcluj.ro. https://orcid.org/0000-0002-0874-9089

Keywords:

androsterone, celery, solid-liquid extraction, detection limits, accuracy

Abstract

Celery is recently the subject of various studies due to its role in human nutrition and for medicine purposes. One of the most speculated ideas is that celery contains high quantities of androsterone which makes it suitable for infertility treatments. Due to this trend, the purpose of this study was to develop an analytical method suitable to confirm and measure the quantity of androsterone from celery root. A LC-MS/MS method was developed using a Turbo V source in positive ionization mode. Analytical parameters such as: linearity, detection and quantification limits, accuracy and precision and matrix effect were evaluated. The calibration curve was developed in the range of 100 to 400 ng/ml with a correlation coefficient r² of 0.9968 and detection limit of 10 ng/ml. The extraction method was tested for the recovery degree. The recovery obtained was 92.1±2.2%. The method was used to determine the content of androsterone from three celery varieties from Romanian market.

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STUDIA UBB CHEMIA, Volume 61 (LXI), No. 3, September 2016

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Published

2016-09-30

How to Cite

SIMEDRU, D. ., NAGHIU, A. ., CADAR, O. ., DORDAI, M. L. ., LUCA, V.-E. ., & SIMON, I. . (2016). DETERMINATION OF ANDROSTERONE FROM CELERY BY A NEW VALIDATED LC-MS/MS METHOD. Studia Universitatis Babeș-Bolyai Chemia, 61(3), 415–422. Retrieved from https://studia.reviste.ubbcluj.ro/index.php/chemia/article/view/8364