SPIN LABELLED HEMOGLOBIN-BASED OXYGEN CARRIERS (HBOC): PREPARATION AND EVALUATION OF IN VIVO / IN VITRO STABILITY

Radu SILAGHI-DUMITRESCU; Cristina GRUIAN; Cristina PUȘCAȘ; Alexandra SIMON; Eva FISHER-FODOR; Vlad A. TOMA; Anca  FĂRCAȘ; Ioana  ROMAN; Violeta-Florina SCURTU; Amr A.A. ATTIA; Grigore  DAMIAN

doi:10.24193/subbchem.2020.2.10

SPIN LABELLED HEMOGLOBIN-BASED OXYGEN CARRIERS (HBOC): PREPARATION AND EVALUATION OF IN VIVO / IN VITRO STABILITY

Authors

Radu SILAGHI-DUMITRESCU Professor PhD, Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca; Romanian Chemical Society, Romanian Society of Biochemistry and Molecular Biology, Society of Biological Inorganic Chemistry, Romania. *Corresponding author: rsilaghi@chem.ubbcluj.ro. https://orcid.org/0000-0003-3038-7747
Cristina GRUIAN Institute for Interdisciplinary Research on Bio-Nano-Systems, Babes-Bolyai University, Cluj-Napoca, Romania. https://orcid.org/0000-0002-7932-5590
Cristina PUȘCAȘ Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania. https://orcid.org/0000-0002-6910-8816
Alexandra SIMON Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania. https://orcid.org/0000-0002-1193-9246
Eva FISHER-FODOR Ion Chiricuta Cancer Institute, Comprehensive Cancer Center, Cluj-Napoca, Romania.
Vlad A. TOMA Institute of Biological Research, Babes-Bolyai University; National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania. https://orcid.org/0000-0002-1141-2559
Anca FĂRCAȘ Institute of Biological Research, Babes-Bolyai University; National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania. Email: ancuta.farkas@ubbcluj.ro. https://orcid.org/0000-0002-7085-1623
Ioana ROMAN Institute of Biological Research, Babes-Bolyai University, Cluj-Napoca, Romania. Email: ioana.roman@usamvcluj.ro. https://orcid.org/0000-0001-8002-8035
Violeta-Florina SCURTU Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania. https://orcid.org/0000-0002-2406-9163
Amr A.A. ATTIA Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania. https://orcid.org/0000-0001-7020-5691
Grigore DAMIAN Department of Physics, Babes-Bolyai University, Cluj-Napoca, Romania. Email: grigore.damian@phys.ubbcluj.ro. https://orcid.org/0000-0003-4127-8140

DOI:

https://doi.org/10.24193/subbchem.2020.2.10

Keywords:

hemoglobin, albumin, blood substitute, HBOC, EPR, spin label, MTSSL, in vivo, cell culture, free radical

Abstract

Site-directed spin labeling and EPR characterization (continuous wave as well as DEER) of two blood substitute candidates is described for the first time: glutaraldehyde-polymerized bovine hemoglobin and the related hemoglobin-albumin copolymer. In vitro, with two different types of cell cultures, these spin-labeled candidates are relatively stable; however, they appear to be rapidly reduced in vivo, most likely via removal or reduction of the spin label (as opposed to removal of the blood substitute candidate from the circulatory system altogether).

References

Klare, J.P.; Steinhoff, H.J. Photosynth. Res. 2009, 102, 377–390.

Horváth, L.I.; Török, M.; Hideg, K.; Dux, L. J. Mol. Recognit. 1997, 10, 188–193.

Berliner, L.J.; Grunwald, J.; Hankovszky, H.O.; Hideg, K. Anal. Biochem. 1982, 119, 450–455.

Hubbell, W.L.; Cafiso, D.S.; Altenbach, C. Nat. Struct. Biol. 2000, 7, 735–739.

Banham, J.E.; Baker, C.M.; Ceola, S.; Day, I.J.; Grant, G.H.; Groenen, E.J. J.; Rodgers, C.T.; Jeschke, G.; Timmel, C.R. J. Magn. Reson. 2008, 191, 202–218.

Grote, M.; Bordignon, E.; Polyhach, Y.; Jeschke, G.; Steinhoff, H.J.; Schneider, E. Biophys. J. 2008, 95, 2924–2938.

Borbat, P.P.; Freed, J.H. Chem. Phys. Lett. 1999, 313, 145–154.

Pannier, M.; Veit, S.; Godt, A.; Jeschke, G.; Spiess, H.W. J. Magn. Reson. 2000, 142, 331–340.

Schiemann, O.; Prisner, T.F. Q. Rev. Biophys. 2007, 40, 1–53.

Klare, J.P. Biol. Chem. 2013, 394, 1281–1300.

Jeschke, G. Annu. Rev. Phys. Chem. 2012, 63, 419–446.

Gruian, C.; Steinhoff, H.J.; Simon, S. Studia UBB Chemia 2011, 71–81.

Gruian, C.; Vulpoi, A.; Steinhoff, H.J.; Simon, S. J. Mol. Struct. 2012, 1015, 20–26.

Takacs, I.M.; Mot, A.; Silaghi-Dumitrescu, R.; Damian, G. J. Mol. Struct. 2014, 1073, 18–23.

Takacs, M.I.; Mot, A.; Silaghi-Dumitrescu, R.; Damian, G. Studia UBB Chemia 2013, 58, 61–69.

Iacob, B.; Deac, F.; Cioloboc, D.; Damian, G.; Silaghi-Dumitrescu, R. Artif. Cells Blood Substitutes Biotechnol. 2011, 39, 293–297.

Scurtu, F.; Zolog, O.; Iacob, B.; Silaghi-Dumitrescu, R. Artif. Cells Nanomedicine Biotechnol. 2014, 42, 13–17.

Hathazi, D.; Scurtu, F.; Bischin, C.; Mot, A.; Attia, A.; Kongsted, J.; Silaghi-Dumitrescu, R. Molecules 2018, 23, E350.

Toma, V.A.; Farcas, A.D.; Roman, I.; Sevastre, B.; Hathazi, D.; Scurtu, F.; Damian, G.; Silaghi-Dumitrescu, R. PLoS One 2016, 11, e0153909.

Hathazi, D.; Mot, A.C.; Vaida, A.; Scurtu, F.; Lupan, I.; Fischer-Fodor, E.; Damian, G.; Kurtz Jr., D.M.; Silaghi-Dumitescu, R. Biomacromolecules 2014, 15, 1920–1927.

Scurtu, V.-F.; Moţ, A.C.; Silaghi-Dumitrescu, R. Pharmaceuticals 2013, 6, 867–880.

Bridges, M.D.; Hideg, K.; Hubbell, W.L. Appl. Magn. Reson. 2010, 37, 363–390.

Pettersen, E.G.; Goddard, T.D.; Huang, C.C.; Couch, G.S.; Greenblatt, D.M.; Meng, E.C.; Ferrin, T.E. J. Comput. Chem. 2004, 25, 1605–1612.

Toma, V.A.A.; Farcas, A.D.D.; Roman, I.; Sevastre, B.; Hathazi, D.; Scurtu, F.; Damian, G.; Silaghi-Dumitrescu, R. Int. J. Biol. Macromol. 2017, 107, 1422–1427.

STUDIA UBB CHEMIA, Volume 65 (LXV), No. 2, June 2020

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Published

2020-06-30

How to Cite

SILAGHI-DUMITRESCU, R., GRUIAN, C., PUȘCAȘ, C., SIMON, A., FISHER-FODOR, E., TOMA, V. A., … DAMIAN, G. . (2020). SPIN LABELLED HEMOGLOBIN-BASED OXYGEN CARRIERS (HBOC): PREPARATION AND EVALUATION OF IN VIVO / IN VITRO STABILITY. Studia Universitatis Babeș-Bolyai Chemia, 65(2), 121–132. https://doi.org/10.24193/subbchem.2020.2.10