SPIN LABELLED HEMOGLOBIN-BASED OXYGEN CARRIERS (HBOC): PREPARATION AND EVALUATION OF IN VIVO / IN VITRO STABILITY

Radu SILAGHI-DUMITRESCU; Cristina GRUIAN; Cristina PUȘCAȘ; Alexandra SIMON; Eva FISHER-FODOR; Vlad A. TOMA; Anca  FĂRCAȘ; Ioana  ROMAN; Violeta-Florina SCURTU; Amr A.A. ATTIA; Grigore  DAMIAN

doi:10.24193/subbchem.2020.2.10

Authors

Radu SILAGHI-DUMITRESCU Professor PhD, Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca; Romanian Chemical Society, Romanian Society of Biochemistry and Molecular Biology, Society of Biological Inorganic Chemistry, Romania. *Corresponding author: rsilaghi@chem.ubbcluj.ro. https://orcid.org/0000-0003-3038-7747 (unauthenticated)
Cristina GRUIAN Institute for Interdisciplinary Research on Bio-Nano-Systems, Babes-Bolyai University, Cluj-Napoca, Romania. https://orcid.org/0000-0002-7932-5590 (unauthenticated)
Cristina PUȘCAȘ Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania. https://orcid.org/0000-0002-6910-8816 (unauthenticated)
Alexandra SIMON Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania. https://orcid.org/0000-0002-1193-9246 (unauthenticated)
Eva FISHER-FODOR Ion Chiricuta Cancer Institute, Comprehensive Cancer Center, Cluj-Napoca, Romania.
Vlad A. TOMA Institute of Biological Research, Babes-Bolyai University; National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania. https://orcid.org/0000-0002-1141-2559 (unauthenticated)
Anca FĂRCAȘ Institute of Biological Research, Babes-Bolyai University; National Institute for Research and Development of Isotopic and Molecular Technologies, Cluj-Napoca, Romania. Email: ancuta.farkas@ubbcluj.ro. https://orcid.org/0000-0002-7085-1623 (unauthenticated)
Ioana ROMAN Institute of Biological Research, Babes-Bolyai University, Cluj-Napoca, Romania. Email: ioana.roman@usamvcluj.ro. https://orcid.org/0000-0001-8002-8035 (unauthenticated)
Violeta-Florina SCURTU Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania. https://orcid.org/0000-0002-2406-9163 (unauthenticated)
Amr A.A. ATTIA Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania. https://orcid.org/0000-0001-7020-5691 (unauthenticated)
Grigore DAMIAN Department of Physics, Babes-Bolyai University, Cluj-Napoca, Romania. Email: grigore.damian@phys.ubbcluj.ro. https://orcid.org/0000-0003-4127-8140 (unauthenticated)

DOI:

https://doi.org/10.24193/subbchem.2020.2.10

Keywords:

hemoglobin, albumin, blood substitute, HBOC, EPR, spin label, MTSSL, in vivo, cell culture, free radical

Abstract

Site-directed spin labeling and EPR characterization (continuous wave as well as DEER) of two blood substitute candidates is described for the first time: glutaraldehyde-polymerized bovine hemoglobin and the related hemoglobin-albumin copolymer. In vitro, with two different types of cell cultures, these spin-labeled candidates are relatively stable; however, they appear to be rapidly reduced in vivo, most likely via removal or reduction of the spin label (as opposed to removal of the blood substitute candidate from the circulatory system altogether).

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