EXAMINATION OF (PHENOTHIAZINYL)VINYL-PYRIDINIUM DYE’S CAPACITY OF INTERACTION WITH DNA

Bianca STOEAN; Luiza GAINA; Emese GAL; Castelia CRISTEA; Tamas LOVASZ; Luminita SILAGHI-DUMITRESCU

doi:10.24193/subbchem.2021.2.05

EXAMINATION OF (PHENOTHIAZINYL)VINYL-PYRIDINIUM DYE’S CAPACITY OF INTERACTION WITH DNA

Authors

Bianca STOEAN Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos str., RO-400028, Cluj-Napoca, Romania. *Corresponding author: sba2488@chem.ubbcluj.ro
Luiza GAINA Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos str., RO-400028, Cluj-Napoca, Romania. https://orcid.org/0000-0002-1328-0107
Emese GAL Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos str., RO-400028, Cluj-Napoca, Romania. https://orcid.org/0000-0003-4324-0787
Castelia CRISTEA Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos str., RO-400028, Cluj-Napoca, Romania. https://orcid.org/0000-0003-1138-2596
Tamas LOVASZ Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos str., RO-400028, Cluj-Napoca, Romania. https://orcid.org/0000-0003-2923-7442
Luminita SILAGHI-DUMITRESCU Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos str., RO-400028, Cluj-Napoca, Romania. https://orcid.org/0000-0001-8800-4839

DOI:

https://doi.org/10.24193/subbchem.2021.2.05

Keywords:

cyanine dyes, phenothiazine, DNA binding, association constant.

Abstract

The interaction of (E)-4-(2-(7-bromo-10-ethyl-10H-phenothiazin-3-yl)vinyl)-1-methylpyridin-1-ium (PVP) dyes with calf-thymus DNA was examined by spectrophotometric UV-vis absorption/emission titration and competitive ethidium bromide displacement. The association constant values were situated in 10⁶ M^-1 range, typical to cationic dyes containing the monocyanine chromophore system. The variation of the counterion of the cationic PVP dye from iodide to tetrafluoroborate produced dissimilarities in the interaction pattern with DNA. The PVP iodide competitively displaced the ethidium bromide (EB) from the EB-DNA system, thus suggesting the intercalation ability of the dye, while the PVP tetrafluoroborate did not supported the intercalation of the dye.

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STUDIA UBB CHEMIA, Volume 66 (LXVI), No. 2, June 2021

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Published

2021-06-30

How to Cite

STOEAN, B., GAINA, L., GAL, E., CRISTEA, C., LOVASZ, T., & SILAGHI-DUMITRESCU, L. (2021). EXAMINATION OF (PHENOTHIAZINYL)VINYL-PYRIDINIUM DYE’S CAPACITY OF INTERACTION WITH DNA. Studia Universitatis Babeș-Bolyai Chemia, 66(2), 59–66. https://doi.org/10.24193/subbchem.2021.2.05