THE COMPLEMENTARY ROLE OF THE RAMAN MICROSPECTROSCOPY TO THE OXIDATIVE STRESS ASSAYS IN THE NEONATAL SYNAPTOSOMES CHARACTERIZATION

Authors

  • Vlad-Alexandru TOMA National Institute for Research and Development of Isotopic and Molecular Technologies, Donath str. No. 67-103, 400293 Cluj-Napoca, Romania; Babeș-Bolyai University, Faculty of Biology and Geology, Clinicilor str. No. 5-7, 400006 Cluj-Napoca, Romania; National Institute of Research and Development for Biological Sciences, Institute of Biological Research, Republicii str. No. 48, 400015 Cluj-Napoca, Romania. https://orcid.org/0000-0002-1141-2559
  • Alia COLNIȚĂ National Institute for Research and Development of Isotopic and Molecular Technologies, Donath str. No. 67-103, 400293 Cluj-Napoca, Romania. https://orcid.org/0000-0002-5795-8928
  • Ioana BREZEȘTEAN National Institute for Research and Development of Isotopic and Molecular Technologies, Donath str. No. 67-103, 400293 Cluj-Napoca, Romania; Babeş-Bolyai University, Faculty of Physics, Kogalniceanu str. No.1, 400084, Cluj-Napoca, Romania. https://orcid.org/0000-0001-6374-5155
  • Bogdan DUME Babeș-Bolyai University, Faculty of Biology and Geology, Clinicilor str. No. 5-7, 400006 Cluj-Napoca, Romania.
  • Ioana ROMAN National Institute of Research and Development for Biological Sciences, Institute of Biological Research, Republicii str. No. 48, 400015 Cluj-Napoca, Romania. https://orcid.org/0000-0001-8002-8035
  • Ioan TURCU National Institute for Research and Development of Isotopic and Molecular Technologies, Donath str. No. 67-103, 400293 Cluj-Napoca, Romania. *Corresponding author: ioan.turcu@itim-cj.ro https://orcid.org/0000-0001-6109-6059

DOI:

https://doi.org/10.24193/subbchem.2021.3.11

Keywords:

Raman, synaptosomes, brain, vesicles, sensitive method, redox status.

Abstract

Raman microspectroscopy was tested as an alternative/complementary method for biochemical evaluation of the synaptosomes obtained from neonatal rat brain prenatally exposed to sodium valproate and treated with allicin. Spectrophotometric assays of several oxidative stress markers (catalase, superoxide dismutase, total thiols) and acetylcholine esterase activity revealed the redox balancing function and pro-cholinergic effect of the allicin as compared to the valproate effect. Raman evaluation showed no significant changes in our experimental conditions. Different concentrations and volumes of the synaptosomes vesicles must be tested for the optimal Raman examination of these purified synaptosomes.

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STUDIA UBB CHEMIA, Volume 66 (LXVI), No. 3, September 2021

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Published

2021-09-30

How to Cite

TOMA, V.-A., COLNIȚĂ, A., BREZEȘTEAN, I., DUME, B., ROMAN, I., & TURCU, I. (2021). THE COMPLEMENTARY ROLE OF THE RAMAN MICROSPECTROSCOPY TO THE OXIDATIVE STRESS ASSAYS IN THE NEONATAL SYNAPTOSOMES CHARACTERIZATION. Studia Universitatis Babeș-Bolyai Chemia, 66(3), 187–194. https://doi.org/10.24193/subbchem.2021.3.11

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Volume 66, No. 3, 2021

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