PREPARATION, PHYSICO-CHEMICAL CHARACTERIZATION AND ANTIBACTERIAL PROPERTIES OF CHITOSAN AND CHITOSAN–NISIN MEMBRANES

Authors

  • Mariana Adina MATICA West University of Timișoara, Advanced Environmental Research Laboratories, 4 Oituz str., RO-300086, Timișoara, Romania. https://orcid.org/0000-0003-1435-6713
  • Finn Lillelund AACHMANN Norwegian Biopolymer Laboratory (NOBIPOL), Department of Biotechnology and Food Sciences, NTNU Norwegian University of Science and Technology, Sem Sælands vei 6/8, 7491 Trondheim, Norway. https://orcid.org/0000-0003-1613-4663
  • Anne TØNDERVIK SINTEF Industry, Department of Biotechnology and Nanomedicine, Richard Birkelands veg 3 B, 7034 Trondheim, Norway
  • Håvard SLETTA SINTEF Industry, Department of Biotechnology and Nanomedicine, Richard Birkelands veg 3 B, 7034 Trondheim, Norway.
  • Vasile OSTAFE West University of Timișoara, Advanced Environmental Research Laboratories, 4 Oituz str., RO-300086, Timișoara, Romania. *Corresponding author: vasile.ostafe@e-uvt.ro https://orcid.org/0000-0003-1352-1115

DOI:

https://doi.org/10.24193/subbchem.2022.1.14

Keywords:

chitosan, membrane, nisin, antimicrobial peptide, biodegradable polymer.

Abstract

Different types of chitosan were used to prepare membranes with enhanced antibacterial properties, via the solvent casting method. Nisin, an antimicrobial peptide, already use in food preservation, was incorporated in chitosan membranes to enhance the bactericidal effect, to obtain a starting material intended for use as wound dressings. The physico-chemical properties of the membranes were monitored and the results showed a good swelling capacity and water vapor transmission rate of the membranes. Optical characterization data showed that chitosan-based membranes could provide ultraviolet light protection while in vitro biodegradability assay demonstrated good stability of the films under enzymatic degradation. Nisin improved significantly the antibacterial effect of the membranes, while the nisin-chitosan membrane-forming solutions had a bactericidal effect against both Gram-positive and Gram-negative bacteria.

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Published

2022-03-30

How to Cite

MATICA, M. A., AACHMANN, F. L., TØNDERVIK, A., SLETTA, H., & OSTAFE, V. (2022). PREPARATION, PHYSICO-CHEMICAL CHARACTERIZATION AND ANTIBACTERIAL PROPERTIES OF CHITOSAN AND CHITOSAN–NISIN MEMBRANES. Studia Universitatis Babeș-Bolyai Chemia, 67(1), 209–226. https://doi.org/10.24193/subbchem.2022.1.14

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