DETERMINATION OF BIOGENIC AMINES BY USING AMPEROMETRIC BIOSENSORS BASED ON GRASS PEA AMINE OXIDASE AND OAT POLYAMINE OXIDASE

Authors

  • Laura AQUILANTE Department of Analytical Chemistry, Lund University, Lund, Sweden; British School of Geneva, Geneva, Switzerland. Corresponding author: cpopescu@chem.ubbcluj.ro.
  • Mihaela NISTOR Department of Analytical Chemistry, Lund University, Lund, Sweden; British School of Geneva, Geneva, Switzerland. Corresponding author: cpopescu@chem.ubbcluj.ro. https://orcid.org/0000-0001-5905-4183
  • Stefania TAORMINA Department of Analytical Chemistry, Lund University, Lund, Sweden; London Health Coaching, United Kingdom. Corresponding author: cpopescu@chem.ubbcluj.ro.
  • Marek ŠEBELA Centre of the Haná Region for Biotechnological and Agricultural Research, Palacký University, Olomouc, Czech Republic. Corresponding author: cpopescu@chem.ubbcluj.ro. https://orcid.org/0000-0001-9835-1375
  • Ivo FRÉBORT Centre of the Haná Region for Biotechnological and Agricultural Research, Palacký University, Olomouc, Czech Republic. Corresponding author: cpopescu@chem.ubbcluj.ro. https://orcid.org/0000-0002-4032-4961
  • Ionel Cătălin POPESCU Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Corresponding author: cpopescu@chem.ubbcluj.ro. https://orcid.org/0000-0002-8700-886X

DOI:

https://doi.org/10.24193/subbchem.2020.3.01

Keywords:

Amine oxidase, polyamine oxidase, amperometric reagentless biosensors, biogenic amines, human urine

Abstract

Grass pea amine oxidase (GPAO) and oat polyamine oxidase (OPAO) were immobilized along with horseradish peroxidase (HRP) and an Os-redox polymer (Os-RP) onto the surface of a graphite electrode by cross-linking with poly(ethylenglycol) diglycidyl ether. The resulted reagentless amperometric biosensors were inserted in a flow injection setup and used as electrochemical detectors for the biogenic amines (BA) detection. Both biosensors were operated at low applied potential (-50 mV vs. Ag/AgCl, KCl0.1M) where electrochemical interferences are minimal. The quantification of ten BA (tyramine, putrescine, cadaverine, histamine, cystamine, phenylethylamine, agmatine, tryptamine, spermine, and spermidine) either individual or in mixture (after a preliminary separation by using cation exchange chromatography) was reported. G/(Os-RP)-HRP-GPAO biosensor detected all ten BA, while G/(Os-RP)-HRP-OPAO biosensor detected only spermine and spermidine. Finally, a simple and low-cost method for free and acetylated polyamines determination in human urine samples, by using the highly selective G/(Os-RP)-HRP-OPAO biosensor, was proposed.

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STUDIA UBB CHEMIA, Volume 65 (LXV), No. 3, September 2020

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2020-09-30

How to Cite

AQUILANTE, L. ., NISTOR, M. ., TAORMINA, S. ., ŠEBELA, M. ., FRÉBORT, I. ., & POPESCU, I. C. . (2020). DETERMINATION OF BIOGENIC AMINES BY USING AMPEROMETRIC BIOSENSORS BASED ON GRASS PEA AMINE OXIDASE AND OAT POLYAMINE OXIDASE. Studia Universitatis Babeș-Bolyai Chemia, 65(3), 9–21. https://doi.org/10.24193/subbchem.2020.3.01

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Volume 65, No. 3, 2020

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