GLYPHOSATE AND AMINOMETHYLPHOSPHONIC  ACID LEVELS IN WATER AND SOIL SAMPLES FROM TRANSYLVANIAN ROMA COMMUNITY ANALYZED  BY HPLC-FLD METHOD

Mihaela VLASSA; Miuța FILIP; Virginia COMAN; Vlad PĂNESCU; Cătălina HERGHELEGIU; Simion BELDEAN-GALEA

doi:10.24193/subbchem.2022.4.18

GLYPHOSATE AND AMINOMETHYLPHOSPHONIC ACID LEVELS IN WATER AND SOIL SAMPLES FROM TRANSYLVANIAN ROMA COMMUNITY ANALYZED BY HPLC-FLD METHOD

Authors

Mihaela VLASSA Babeş-Bolyai University, Raluca Ripan Institute for Research in Chemistry, 30 Fântânele Str., RO-400294, Cluj-Napoca, Romania. mihaela.vlassa@chem.ubbcluj.ro, mihaela_cecilia@yahoo.com. https://orcid.org/0000-0002-8141-2570
Miuța FILIP Babeş-Bolyai University, Raluca Ripan Institute for Research in Chemistry, 30 Fântânele Str., RO-400294, Cluj-Napoca, Romania. miuta.filip@ubbcluj.ro https://orcid.org/0000-0001-6536-7696
Virginia COMAN Babeş-Bolyai University, Raluca Ripan Institute for Research in Chemistry, 30 Fântânele Str., RO-400294, Cluj-Napoca, Romania. virginia.coman@ubbcluj.ro
Vlad PĂNESCU Babeş-Bolyai University, Faculty of Environmental Science and Engineering, 30 Fântânele Str., RO-400294, Cluj-Napoca, Romania. vlad.panescu@ubbcluj.ro
Cătălina HERGHELEGIU Babeş-Bolyai University, Faculty of Environmental Science and Engineering, 30 Fântânele Str., RO-400294, Cluj-Napoca, Romania. mihaela.herghelegiu@ubbcluj.ro
Simion BELDEAN-GALEA Babeş-Bolyai University, Faculty of Environmental Science and Engineering, 30 Fântânele Str., RO-400294, Cluj-Napoca, Romania. simion.beldean@ubbcluj.ro https://orcid.org/0000-0002-6892-1223

DOI:

https://doi.org/10.24193/subbchem.2022.4.18

Keywords:

Glyphosate, aminomethylphosphonic acid, water, soil, FMOC-Cl, RP-HPLC-FLD.

Abstract

This paper purpose was to develop a sensitive and selective method for the determination of glyphosate, and aminomethylphosphonic acid (AMPA) residues in water and glyphosate from soil samples. The method involves a derivatization step with 9-fluorenylmethylchloroformate in borate buffer of these compounds and liquid chromatography separation with fluorescence detection (HPLC-FLD). Separation of derivatized glyphosate and AMPA compounds was performed on an Agilent ZORBAX C18 reversed-phase column. The mobile phase consisted of a mixture of acetonitrile and 0.05 M KH₂PO₄ solution [30:70 v/v]. Limits of detection (LOD) was 0.28 µg L^-1 for glyphosate, and 0.35 µg L^-1for AMPA, and limits of quantification (LOQ) was 0.84 µg L^-1for glyphosate and 1.05 µg L^-1for AMPA. The method has been validated for surface water and soil by recovery studies with samples spiked at 25 and 5 µg L^-1. In water samples, the mean recoveries values ranged between 86.44 - 103.9% for glyphosate, and 71.27 - 99.08% for AMPA. The mean recoveries values for glyphosate ranged from 57 - 81.5% in soil samples. The developed method has been applied for determination of these compounds in water and soil samples from agricultural and rural areas of Roma community from some Transylvania Counties.

References

P. J. Peruzzo; A. A. Porta; A. E. Ronco; Environ. Pollut., 2008, 156, 61-66

I. Freuze; A Jadas-Hecart; A. Royer; P-Y. Communal; J. Chromatogr. A, 2007, 1175, 197-206

M. P. García de Llasera; L. Gómez-Almaraz; L. E. Vera-Avila; A. Peña-Alvarez; J. Chromatogr. A, 2005, 1093, 139–146

H. Karasali; G. Pavlidis; A. Marousopoulou; Environ. Sci. Pollut. Res., 2019, 26, 36308–36321

J. P. Munňoz; T. C. Bleak; G. M. Calaf; Chemosphere, 2021, 270, 128619

L. Battisti; M. Potrich; A. R. Sampaio; N. de Castilhos Ghisi; F. M. Costa-Maia; R Abati; C.B. Dos Reis Martinez; S.H.Sofia; Sci. Total Environ., 2021, 767, 145397

Y. Sang; J.-C. Mejuto; J. Xiao;J. Simal-Gandara; Plants, 2021, 10, 405-427

H. Vereecken; Pest Manag. Sci., 2005, 61, 1139–1151

C. P. Bento; X. Yang; G. Gort; S. Xue; R. van Dam; P. Zomer; H. G. J. Mol; C. J. Ristema; V. Geissen; Sci. Total Environ., 2016, 572, 301–311

T. Arkan; I. Molnár-Perl; Microchem. J., 2015, 121, 99–106

L. J. Marek; W. C Koskinen; Pest Manag. Sci., 2014, 70, 1158-1164

L. B. Bhaskara; P. Nagaraja; Helv. Chim. Acta, 2006, 89, 2686-2693

M. E. Baez; E. Fuentes; M. J. Espina; J. Espinoza; J. Sep. Sci. 2014, 37, 3125-3132

K. Mardian-Jansar; B. S. Ismail; AIP Conference Proceedings, 2014, 1614, 795-802

S. Wang; B. Liu, D. Yuan; J. Ma; Talanta, 2016, 161, 700–706

L. Sun; D. Kong; W. Gu; X. Guo; W. Tao; Z. Shan; Y. Wang; N. Wang; J. Chromatogr. A, 2017,1502, 8–13

J. Garba; A. W. Samsuri; R. Othman; M. S. A. Hamdani; Environ. Sci. Technol., 2018, 1, 19-30

T. C. P. G. Catrinck; A. Dias; M. C. S. Aguiar; F. O. Silvério; P. H. Fidêncio; G. P. Pinho; Braz. Chem. Soc., 2014, 25, 1194–1199

W. Skeff; C. Recknagel; D. E. Schulz-Bull; J. Chromatogr. A, 2016, 1475, 64–7310-11.

A. M. Botero-Coy; M. Ibanez; J. V. Sancho; F. Hernandez; J. Chromatogr. A, 2013; 1292, 132-141

L. J. Marek; W. C. Koskinen; Pest Manag. Sci., 2014, 70(7), 1158-1164

L. L. Alonso; P. M. Demetrio; M. A. Etchegoyen; D. J. Marino; Sci. Total Environ., 2018, 645, 89-96

T. Erban; M. Stehlik; B. Sopko; M. Markovic; M. Seifrtova; T. Halesova; P. Kovaricek; Chemosphere, 2018, 207, 78-83

M. Ibáñez; Ó. J. Pozo; J. V. Sancho; F. J. López; F. Hernández; J. Chromatogr. A, 2005, 1081, 145-1557

A. Ghanem; P. Bados; L. Kerhoas; J. Dubroca; J. Einhorn; Anal. Chem. 2007; 79, 3794-3801

T. Ohara; T. Yoshimoto; Y. Natori; A. Ishii; Nagoya J. Med. Sci., 2021, 83, 567-587

V. C. Aparicio; E. De Gerónimo, D. Marino; J. Primost; P. Carriquiriborde; J. L. Costa; Chemosphere, 2013, 93, 1866–1873

M. Popp; S. Hann; A. Mentler; M. Fuerhacker; G. Stingeder; G. Koellensperger; Anal. Bioanal. Chem., 2008, 391, 695–699

J. P. F. Tiago; L. C. Sicupira; R. E. Barros; G. P. de Pinho; F. O. Silvério; J. Environ. Sci. Health - B Pestic. Food Contam. Agric. Wastes, 2020, 1532-4109

V. G. Amelina; A. M. Andoralov; J. Anal. Chem., 2016, 71, 82–93

ICH harmonised tripartite guideline. Validation of analytical procedures: Text and methodology Q2 (R1). 2005. http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1_Guideline.pdf, pp. 1-17.]

T. V. Nedelkoska; G. K-C. Low; Anal. Chim. Acta, 2004, 511, 145–153

J. E. Primost; D. J. Marino; V. C. Aparicio; J. L. Costa; P. Carriquiriborde; Environ Pollut. 2017, 229, 771–779.

STUDIA UBB CHEMIA, Volume 67 (LXVII), No. 4, December 2022

Downloads

Published

2022-12-30

How to Cite

VLASSA, M., FILIP, M., COMAN, V., PĂNESCU, V., HERGHELEGIU, C., & BELDEAN-GALEA, S. (2022). GLYPHOSATE AND AMINOMETHYLPHOSPHONIC ACID LEVELS IN WATER AND SOIL SAMPLES FROM TRANSYLVANIAN ROMA COMMUNITY ANALYZED BY HPLC-FLD METHOD. Studia Universitatis Babeș-Bolyai Chemia, 67(4), 273–285. https://doi.org/10.24193/subbchem.2022.4.18