Determination of Chemical Compositions, Antioxidant, DNA Cleavage and Binding Properties of Vincetoxicum Tmoleum Extract

Authors

  • Emine KILIÇKAYA SELVI Department of Chemistry and Chemical Processing Technologies, Mustafa Çıkrıkcıoglu Vocational School, Kayseri University, Kayseri, Türkiye. *Corresponding author: emineselvi@kayseri.edu.tr https://orcid.org/0000-0003-0291-5362
  • Nilgün GÜLER Department of Chemistry and Chemical Processing Technologies, Mustafa Çıkrıkcıoglu Vocational School, Kayseri University, Kayseri, Türkiye. https://orcid.org/0000-0003-3022-4876
  • Seher GÜVEN Department of Biology, Faculty of Sciences and Arts, Recep Tayyip Erdogan University, Rize, Türkiye.
  • Serdar MAKBUL Department of Biology, Faculty of Sciences and Arts, Recep Tayyip Erdogan University, Rize, Türkiye. https://orcid.org/0000-0001-8798-0926

DOI:

https://doi.org/10.24193/subbchem.2024.1.10

Keywords:

Vincetoxicum tmoleum, antioxidant activity, DNA cleavage, HPLC-DAD

Abstract

In the present study, phenolic compounds, antioxidant activities, DNA cleavage and binding effects of Vincetoxicum tmoleum (Vt) collected from west of Anatolia (Manisa city) were investigated. Antioxidant potentials of the extracts were characterized with their total phenolic and flavonoid contents, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and FRAP (ferric reducing antioxidant power assay) tests. Besides, the DNA cleavage and binding features of V. tmoleum extract were studied using pBR322 DNA and CT-DNA, respectively. Phenolic compounds of the extract were analyzed by high performance liquid chromatography (HPLC-DAD). As a result, V. tmoleum methanol extract was found to have the high total phenolic and total flavonoid content and antioxidant effect. Strong positive correlations were also found between DPPH and TFC (r = 0.995; p < 0.01), and TPC (r = 0.989; p < 0.01), ABTS (r = 0.994;p < 0.01) and FRAP(r=0,995; p < 0.01). Methanol extract of V. tmoleum had large amounts of p-coumaric acid, ferulic acid and protocatechuic acid. While V. tmoleum samples showed weak DNA cleavage activity, they showed DNA binding activity at 50 µM concentration, that is, the potential to be an intercalation agent in this concentration.

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Published

2024-03-30

How to Cite

KILIÇKAYA SELVI, E., GÜLER, N., GÜVEN, S., & MAKBUL, S. (2024). Determination of Chemical Compositions, Antioxidant, DNA Cleavage and Binding Properties of Vincetoxicum Tmoleum Extract. Studia Universitatis Babeș-Bolyai Chemia, 69(1), 163–174. https://doi.org/10.24193/subbchem.2024.1.10

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