Photoperiodic influence of light-emitting diode (LED) on vegetative parameters of Spinacia oleracea L. (Spinach)

Authors

  • Musa SAHEED IBRAHIM Department of Biology and Forensic Science, Admiralty University of Nigeria, Delta State Nigeria; Department of Plant Biology and Biotechnology, University of Benin, Edo State Nigeria. https://orcid.org/0000-0001-6875-6929
  • Beckley IKHAJIAGBE Department of Plant Biology and Biotechnology, University of Benin, Edo State Nigeria. https://orcid.org/0000-0003-2834-7447
  • Kingsley Erhons ENERIJIOFI Department of Biological Sciences, College of Basic, Applied and Health Sciences, Glorious Vision University, Ogwa, Edo State Nigeria. ✉Corresponding author Email: kingsleyenerijiofi@gmail.com https://orcid.org/0000-0001-7513-4052

DOI:

https://doi.org/10.24193/subbbiol.2025.2.05

Keywords:

breeding chambers, light-emitting diode, photoduration, photoperiodic influence

Abstract

This research aims to determine sustainable strategies to optimize crop growth and yield, by testing the possibilities of using light-emitting diode (LED) technique to influence the vegetative parameters of spinach. A speed-breeding chamber was constructed using LEDs as a light source under varying photoperiods (19, 17, 15, and 13 hours). The control was established to be the normal light duration of 11 hours during the study period. Spinach vegetative parameters involving morphological parameters such as stem length, root length, and leaf area as well as physiological parameters such as plant weight and percentage necrosis and chlorosis were investigated for 30 days after transplanting. The results showed a significant (p < 0.05) increase in morphological parameters of spinach with increasing photoperiod. The spinach plant under the long photoperiod was observed to show the highest morphological and physiological properties. About a 30% increase in root length was observed in the speed-breeding chamber with the longest photoperiod duration compared to the control conditions. Significantly improved spinach plant weight was observed for 19-hour photoperiod compared to the shorter exposure (p < 0.05). A lower percentage of necrosis and chlorosis was observed in spinach with longer LED exposure. This research indicated that LED-induced speed breeding is very effective in improving the vegetative properties of spinach. It can be argued that
a 19-hour LED-induced photoperiod is the optimum photo duration required by spinach to improve vegetative growth. Future research should be conducted to investigate the influence of similar LED-induced photoperiods on other species of vegetables.

 Article history: Received 05 October 2025; Revised 09 December 2025;
Accepted 11 December 2025; Available online 20 December 2025

References

Alika, J. (2006). Statistics and research methods. 2nd Ed. Ambik Press, Benin City, Nigeria. pp. 369. https://doi.org/10.1101/2020.09.30.320952

Arnon, D. (1949). Copper enzymes in isolated chloroplast Polyphenol Oxidase in Beta vulgaris. Plant Physiology. 24, 1-15. https://doi.org/10.1104/pp.24.1.1

Chiurugwi, T., Kemp, S., Powell, W., Hickey, L.T & Powell, W. (2018). Speed breeding orphan crops. Theory of Applied Genetics. 2(2), 12-19. https://doi.org/10.1007/s00122-018-3202-7

Collard, B. C., Beredo, J. C., Lenaerts, B., Mendoza, R., Santelices, R., Lopena, V., Verdeprado, H., Raghavan, C., Gregorio, G. B. & Vial, L. (2017). Revisiting rice-breeding methods evaluating the use of rapid generation advance (RGA) for routine rice breeding. Plant Product of Science. 20, 337–352. https://doi.org/10.1080/1343943x.2017.1391705

Dong, C., Fu, Y., Liu, G. and Liu & H. (2014). Growth, photosynthetic characteristics, antioxidant capacity and biomass yield and quality of wheat (Triticum aestivum L.) exposed to LED light sources with different spectra combinations. Journal of Agronomy and Crop Science. 200, 219–230. https://doi.org/10.1111/jac.12059

Enerijiofi, K. E., Musa, S. I., Igiebor, F. A., Lawani, M., Chuka, N. E. Odozi, E. B. & Ikhajiagbe, B. (2024). Nanotechnological approaches for enhanced microbial activities, sustainable agriculture and the environment. In: Sustainable Agriculture Nanotechnology and Biotechnology for Crop Production and Protection, Rajput, V. D., Singh, A., Ghazaryan, K. and Minkina, T. M. (eds), Walter de Gruyter, Berlin, Germany, pp. 153-183. https://doi.org/101515/978311123494-009

Galvão, V.C. & Fankhauser, C. (2015). Sensing the light environment in plants: Photoreceptors and early signaling steps. Current Opinions in Neurobiology 34, 46-53. https://doi.org/10.1016/j.conb.2015.01.013

Hogewoning, S. W., Trouwburst, G., Maljaars, T. & Pooter, H. (2010). Blue light dose responses of leaf photosynthesis morphology and chemical composition of Cucunis sativus grown under different combinations of red and blue light. Journal of African Breeding. 23(2), 12-23. https://doi.org/10.1093/jxb/erq132

Hussain, H. A., Hussain, S., Khaliq, A., Ashraf, U., Anjum, S. A., Men, S. & Wang, L. (2018). Chilling and drought stresses in crop plants: Implications, crosstalk, and potential management opportunities. Frontiers in Plant Science. 9, 393-394. https://doi.org/10.3389/fpls.2018.00393

Ikhajiagbe, B., Edokpolor, Geoffrey O., Gloria O. & Thomas, U. (2017) Investigating plant growth and physiological response to soil wetting with grey water under different shade regimes: A case of fluted pumpkin (Telfairia occidentalis). Electronic Journal of Polish Agricultural Universities 20 (4), 44-49. https://doi.org/10.30825/5.ejpau.31.2017.20.4

Khan, A., Najeeb, U., Wang, L., Tan, D. K. Y., Yang, G., Munsif, F., Ali, S. & Hafeez, A. (2017). Planting density and sowing date strongly influence growth and lint yield of cotton crops. Field Crops Research. 209, 129–135. https://doi.org/10.1016/j.fcr.2017.04.019

Maxwell, K. & Johnson, G, (2000). Chlorophyll fluorescence - A practical guide, Journal of Experimental Botany. 51, 659-668. https://doi.org/10.1093/jexbot/51.345.659

Monostori, I., Heilmann, M., Kocsy, G., Rakszegi, M., Ahres, M., Altenbach, S. B., Szalai, G., Pál, M., Toldi, D., Simon-Sarkadi, L., Harnos, N., Galiba, G., & Darko, É. (2018). LED lighting - Modification of growth, metabolism, yield and flour composition in wheat by spectral quality and intensity. Frontiers in Plant Science, 9, 605. https://doi.org/10.3389/fpls.2018.00605

Moses Nathan, Ezenwata, I.S. and Musa, S.I. (2023). Some physicochemical properties and heavy metals in water from Oboshi rivee, Ibusa in Delta State, Nigeria. Journal of Applied Sciences and Environmental Management. 27(6), 91-98. https://doi.org/10.4314/jasem.v27i6.33

Musa, S. I. & Ikhajiagbe, B. (2021). The growth response of rice (Oryza sativa L. var. FARO 44) in vitro after inoculation with bacterial isolates from a typical ferruginous ultisol. Bulletin of the National Research Centre. 2(3), 23-29. https://doi.org/10.1186/s42269-021-00528-8

Musa, S.I. and Beckley Ikhajiagbe (2024a). Evaluation of previously isolated and characterized phosphate solubilizing bacteria with plant growth promoting potentials for Rice grown in ferruginous ultisol soil in Benin City, Edo State, Nigeria. J. Appl. Science. Envion. Management. 28, 4393-4407. https://doi.org/10.4314/jasem.v28i12.55

Musa, S.I. and Beckley Ikhajiagbe (2024b). Effects of plant growth promoting bacteria (PGPB) rhizo-inoculation on soil physico-chemical, bacterial community structure and root colonization of rice (Oryza sativa L. var. FARO 44) grown in ferruginous ultisol conditions. Science World Journal. 19(3), 894-899. https://dx.doi.org/10.4314/swj.v19i3.38

Musa, S.I. and Ikhajiagbe B. (2023). Seed bio-priming with phosphate solubilizing bacteria strains to improve rice (Oryza sativa L. var. FARO 44) growth under ferruginous ultisol conditions. BioTechnologia. 27, 33-51. https://doi.org/10.5114/bta.2023.125084

National Research Council. (2006). Assessment of NASA's Mars architecture. The National Academies Press, Washington DC.

Piovene, C., Orsini, F., Bosi, S. & Sanourser, R. (2015). Optimal red: blue ration in led lighting for nutraceutical indoor horticulture. Scientific Horticulture. 193, 202-208. https://doi.org/10.1016/j.scienta.2015.07.015

Priyadarshan, P. M. (2019). Breeding self-pollinated crops. Plant breeding: Classical to Modern. Springer. 23, 12-23.https://doi.org/10.1007/978981-13-7095-3

Rahman, M. A., Quddus, M. R., Jahan, N., Rahman, A., Hossain, M. R. A. S. H. & Iftekharuddaula, K. M. (2019). Field rapid generation advance: An effective technique for industrial scale rice breeding program. The Experiment. 47(2), 2659 2670.https://doi.org/10.1080/1343943X.2017.1391705

Ray, D. K., Mueller, N. D., West, P. C. & Foley, J. A. (2013). Yield trends are insufficient to double global crop production by 2050. Journal of Agricultural Sciences. 12, 23-29. https://doi.org/10.1371/journal.pone.0066428

Rouphael, Y., Cardarelli, M., Schwarz, D., Franken, P. & Colla, G. (2012). Effects of drought on nutrient uptake and assimilation in vegetable crops: In: Plant responses to drought stress, from morphological to molecular features, Aroca R. (ed.), Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32653-0_7

Wolter, F., Schindele, P. & Puchta, H. (2019). Plant breeding at the speed of light: The power of CRISPR/Cas to generate directed genetic diversity at multiple sites. BMC Plant Biology. 19, 1–8. https://doi.org/10.1186/s12870-019-1775-1.

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Published

2025-12-20

How to Cite

SAHEED IBRAHIM, M., IKHAJIAGBE, B., & ENERIJIOFI, K. E. (2025). Photoperiodic influence of light-emitting diode (LED) on vegetative parameters of Spinacia oleracea L. (Spinach). Studia Universitatis Babeș-Bolyai Biologia, 70(2), 69–82. https://doi.org/10.24193/subbbiol.2025.2.05

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