RP-HPLC AND HPTLC-BASED PHYTOCHEMICAL STUDIES OF ENDEMIC NEPETA CADMEA BOISS. AND ITS EFFECTS ON CARBOHYDRATE DIGESTIVE ENZYMES
DOI:
https://doi.org/10.24193/subbchem.2025.4.15Keywords:
α-amylase, α-glucosidase, RP-HPLC, HPTLC, Nepeta cadmeaAbstract
This study investigated the in vitro inhibitory potential of the methanol extract of the aerial parts of Nepeta cadmea on α-glucosidase and α-amylase, which are known as key enzymes related to type 2 diabetes mellitus. To determine the active constituents of the endemic Lamiaceae plant, validated RP-HPLC and HPTLC techniques were used. The methanol extract demonstrated moderate and dose-dependent inhibitory activity, with inhibition rates of 43.57 ± 4.32% against α-glucosidase at 2 mg/mL and 36.74 ± 7.23% against α-amylase at 1 mg/mL. Phytochemical analysis revealed that rosmarinic acid (0.6290 ± 0.0095 g/100g dw) was the predominant phenolic compound, followed by chlorogenic acid (0.0429 ± 0.0012 g/100g dw) and caffeic acid (0.0027 ± 0.0002 g/100g dw). These findings suggest that this endemic Lamiaceae plant may have potential as a natural therapeutic agent for managing diabetes mellitus. Further studies are warranted to explore its mechanisms of action and to evaluate its efficacy in clinical settings.
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