HPLC/DAD ANALYSIS OF VITAMIN C AND ANTIOXIDANT CAPACITY DETERMINATION OF VITIS VINIFERA L. GRAPES DURING RIPENING

Authors

  • Nicoleta MATEI Ovidius University of Constanta, Faculty of Applied Sciences and Engineering, Department of Chemistry and Chemical Engineering, 124 Mamaia Blvd., RO-900527, Constanta, Romania, nmatei@univ-ovidius.ro https://orcid.org/0000-0001-5566-8364
  • Semaghiul BIRGHILA Ovidius University of Constanta, Faculty of Applied Sciences and Engineering, Department of Chemistry and Chemical Engineering, 124 Mamaia Blvd., RO-900527, Constanta, *sbirghila@univ-ovidius.ro https://orcid.org/0000-0002-8829-9101
  • Simona DOBRINAS Ovidius University of Constanta, Faculty of Applied Sciences and Engineering, Department of Chemistry and Chemical Engineering, 124 Mamaia Blvd., RO-900527, Constanta, sdorbinas@univ-ovidius.ro. https://orcid.org/0000-0002-9971-6134
  • Elena Carmen LUPU Ovidius University of Constanta, Faculty of Pharmacy, 6 Căpitan Aviator Al. Șerbănescu Street, Constanta, Romania 900470, clupu@univ-ovidius.ro. https://orcid.org/0000-0002-0200-0157
  • Antoanela POPESCU Ovidius University of Constanta, Faculty of Pharmacy, 6 Căpitan Aviator Al. Șerbănescu Street, Constanta, Romania 900470, antoanela.popescu@univ-ovidius.ro. https://orcid.org/0000-0002-8462-9564

DOI:

https://doi.org/10.24193/subbchem.2025.4.12

Keywords:

Ascorbic acid, grapes, peel, pulp, seed, HPLC-DAD, antioxidant capacity

Abstract

This study aims to determine the vitamin C (ascorbic acid, AA) content and antioxidant capacity in five varieties of grapes (skin, pulp and seeds). A simple, fast and sensitive High–Performance Liquid Chromatography/Diode Array Detection (HPLC-DAD) method was used for the determination of ascorbic acid in grapes (Vitis vinifera L.) samples from Murfatlar vineyard. Total antioxidant capacity was determined through photochemiluminiscence method as ACL (Antioxidant capacity of lipid soluble substances). The LOD was 0.40 µg/mL for the HPLC-DAD method. The advantages of the method are using of small amounts of samples and reagents, short analysis time and minimum steps for sample preparation. Antioxidant capacity of Vitis vinifera L. grapes alcoholic extract ranged between 0.02 and 7.42 mmoles equivalent TROLOX/100g product. After statistical analysis (ANOVA), a significant correlation between the ascorbic acid concentration and the antioxidant capacity was observed in peel, pulp, and seeds during ripening. Due to the high content of ascorbic acid and antioxidant capacity, future analysis should focus on the determination of other bioactive substances in grapes.

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STUDIA UBB CHEMIA, Volume 70 (LXX), No. 4, December 2025

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Published

2025-12-16

How to Cite

MATEI, N., BIRGHILA, S., DOBRINAS, S., LUPU, E. C., & POPESCU, A. (2025). HPLC/DAD ANALYSIS OF VITAMIN C AND ANTIOXIDANT CAPACITY DETERMINATION OF VITIS VINIFERA L. GRAPES DURING RIPENING. Studia Universitatis Babeș-Bolyai Chemia, 70(4), 219–232. https://doi.org/10.24193/subbchem.2025.4.12

Issue

Volume 70, No. 4, 2025

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