A SENSITIVE ANALYTICAL (RP-HPLC-PDA, UV/VIS) METHOD FOR THE DETERMINATION OF NEWLY SYNTHESIZED N-ISONICOTINOYL-N´-(3-FLUOROBENZAL)HYDRAZONE (SH2) IN AQUEOUS PHASE

Zvezdelina  YANEVA; Nedyalka  GEORGIEVA

doi:10.24193/subbchem.2017.2.15

Authors

Zvezdelina YANEVA Chemistry Unit, Department of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria. Email: z.yaneva@abv.bg. https://orcid.org/0000-0001-8159-4530
Nedyalka GEORGIEVA Chemistry Unit, Department of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria. Email: nvgeorgieva@vmf.uni-sz.bg. https://orcid.org/0000-0002-8959-3775

DOI:

https://doi.org/10.24193/subbchem.2017.2.15

Keywords:

N-isonicotynoyl-N´-(3-fluorobenzal)hydrazone, RP-HPLC-PDA, UV/VIS, aqueous phase

Abstract

The purpose of the present study was to develop a simple, rapid and reproducible analytical method for the determination of N-isonicotynoyl-N´-(3-fluorobenzal)hydrazone (SH2) - a synthesized by us halogenated isoniazid derivative with high tuberculostatic activity, in aqueous phase, on the basis of RP-HPLC-PDA and UV/VIS spectrophotometric investigations. Despite of the high linearity (R² 0.9984) of the UV/VIS spectrophotometric method applied, the significantly higher LOQ and LOD values indicated its unsuitability for detection and quantification of low N-isonicotynoyl-N´-(3-fluorobenzal)hydrazone concentrations (< 10 µg/mL). The proposed RP-HPLC-PDA method with mobile phase ACN/phosphate buffer (60:40, v/v) offered short retention time (3.1 min), high precision (RSD 3.50 %) and linearity (R² 0.9898). It characterized with satisfactory LOD (0.346 µg/mL) and LOQ (1.05 µg/mL) values and allowed the qualitative detection of SH2 E/Z-isomer.

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