POLYPHENOLS PROFILE AND ANTIOXIDANT ACTIVITY OF SOME ROMANIAN RANUNCULUS SPECIES

Authors

  • Teodora NEAG Faculty of Pharmacy, Vasile Goldiș Western University, Arad, Romania. Corresponding author: olah.neli@uvvg.ro.
  • Claudia-Crina TOMA Faculty of Pharmacy, Vasile Goldiș Western University, Arad, Romania. Email: claudiatoma2004@yahoo.com. https://orcid.org/0000-0003-1515-3256
  • Neli-Kinga OLAH Faculty of Pharmacy, Vasile Goldiș Western University, Arad; SC PlantExtrakt SRL, Rădaia, Cluj, Romania. Email: olah.neli@uvvg.ro. https://orcid.org/0000-0001-5204-6885
  • Aurel ARDELEAN Department for Cell and Molecular Biology, Faculty of Medicine, "Vasile Goldiş" Western University, Arad; "Vasile Goldiş" Institute of Life Sciences, Western University, Arad; Academy of Medical Sciences of Romania, Cluj-Napoca, Romania. Corresponding author: olah.neli@uvvg.ro.

DOI:

https://doi.org/10.24193/subbchem.2017.3.06

Keywords:

Ranunculus ficaria, Ranunculus bulbosus, Ranunculus sardous, Ranunculus sceleratus, antioxidant activity, total flavonoids and total phenolic acids content, rutoside

Abstract

The Ranunculus species are mostly known for their toxic effect due to the anemonine and protoanemonine content. This paper studies the polyphenols and the antioxidant activity of four different spontaneous species from Ranunculus genus (Ranunculaceae family) harvested from Western Romania’s spontaneous flora. The polyphenols profile was established by TLC and UV-Vis spectrophotometric methods. The antioxidant capacity was evaluated by different in vitro methods: DPPH, TEAC, FRAP, CUPRAC and SNP. From the studied species were prepared two different extracts: hydroalcoholic extracts (HA) respectively glycerol-ethanol extracts (GE). The study indicates that the highest total flavonoid content is in R. ficaria herb HA extract (23 % mg/ml), while the total phenolic acids are the highest in R. bulbosus herb GE extract (14,88 % mg/ml). The most important antioxidant activity was observed at GE extracts obtained from herb of R. ficaria, R. sardous and R. bulbosus. With the less antioxidant effect are the R. sceleratus extracts.

References

A. Ardelean, G.Mohan, “Medicinal Flora of Romania”, Ed. ALL, Bucuresti, 2008, 33.

L. Tongjian, X. Lingling, L. Liang, D. Huisheng, H. Xingjie, Botanical Journal of the Linnean Society of London, 2014, 174 (2), 227.

W. C. Evans In: “Pharmacognosy”, 14th Ed. WB Pourrat H., Texier O. and Regerat F. (1982), Use of an Saunders Company, Tronto- Sydney-Tokyo, 1996.

J. Bruneton, „Pharmacognosie: Phytochimie, Plantes médicinales”, 2nd edition, Paris, 1993, 55.

N. Hadaruga, Chemistry Central Journal, 2012, 762.

S. Aslam, A. Bashir, M. Uzair, A. Subhan, International Journal of Pharmacy and Pharmaceutical Sciences, 2012, 4(5),15.

I. Daraban, “Diversity, bio-economic potential and preservation of flora and halophile vegetation in Campia Aradului”, PhD thesis abstract, 2013.

H. Mei, S. Zuo, L. Ye, J. Wang S. Ma, Journal of Medicinal Plants Research, 2012, 6(10), 1821.

M.N. Alam, N.J. Bristi, M. Rafiquzzaman, Saudi Pharmaceutical Journal, 2013, 21, 143.

K. Thaipong, U. Boonprakob, K. Crosby, L. Cisneros-Zevallos, D. Hawkins Byrne, Journal of Food Composition and Analysis, 2006, 19, 669.

I.F.F. Benzie, J.J Strain., Analytical Biochemistry, 1996, 239, 70.

M.B Arnao., A.Cano, M Acosta, Food chemistry, 2001, 73, 239.

M.Ozyurek, N. Gungor, S. Baki, K. Guclu, R. Apak, Analytical Chemistry, 2012, 84(18), 8052.

“Romanian Pharmacopoeia”, Xth ed., Medical Publishing House Bucharest, Romania, 1993, 335.

“Romanian Pharmacopoeia”, IXth ed., Medical Publishing House Bucharest, Romania, 1976.

S. Cobzac, G. Cimpan, N. Olah, S. Gocan, Journal of Planar Chromatography – Modern TLC, 1999, 12(1), 26.

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Published

2017-09-29

How to Cite

NEAG, T. ., TOMA, C.-C. ., OLAH, N.-K. ., & ARDELEAN, A. (2017). POLYPHENOLS PROFILE AND ANTIOXIDANT ACTIVITY OF SOME ROMANIAN RANUNCULUS SPECIES. Studia Universitatis Babeș-Bolyai Chemia, 62(3), 75–88. https://doi.org/10.24193/subbchem.2017.3.06

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