THE ASSESSMENT OF FLUOROPHORES ADVANCED GLYCATION END PRODUCTS-TO-KYNURENINE RATIO IN HEALTHY AND DIABETIC RATS AND HUMANS

Loredana Elena  OLAR; Dana Mihaela  CIOBANU; Florica  MATEI; Ionel  PAPUC

doi:10.24193/subbchem.2018.1.03

Authors

Loredana Elena OLAR Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Email: loredana.olar@usamvcluj.ro. https://orcid.org/0000-0002-1906-0789
Dana Mihaela CIOBANU Department of Diabetes, Nutrition and Metabolic Diseases, Faculty of Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania. Email: dana.ciobanu@umfcluj.ro. https://orcid.org/0000-0003-3351-6110
Florica MATEI Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Email: faldea@usamvcluj.ro. https://orcid.org/0000-0003-1119-1677
Ionel PAPUC Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Email: papuc_i@yahoo.com. https://orcid.org/0000-0002-2134-8757

DOI:

https://doi.org/10.24193/subbchem.2018.1.03

Keywords:

Advanced glycation end products, Kynurenine, Blood serum, Diabetes Mellitus, Fluorescence Spectroscopy

Abstract

In this study, we calculated the ratio of the serum contributions of two highly studied fluorophore products (advanced glycation end products - AGEs and kynurenine - KYN) in the area of nutrition and metabolic diseases, including diabetes mellitus (DM), by using a non-invasive, economical and easy-to-perform method. Blood serum spectrofluorimetric analysis was performed both in the case of normoglycemic (n=10) and diabetic rats (n=10), and in the case of non-diabetic (n=14) and type 2 diabetes mellitus (T2DM) patients (n=52). Our results showed a significant increase in the contributions of the two products in diabetic patients and rats compared to the control group. The ratio of the two compounds was positively correlated with serum glucose levels in the case of rats, and with serum triglyceride values in the case of humans. Also, the presence of DM complications in human subjects and the subsequent calculation of ROC curves led to a predictive value of the investigated ratio (AGEs/KYN) for the presence of peripheral diabetic polyneuropathy (PNP). The obtained results suggest a high potential for the investigated ratio to be considered as a new biomarker for the presence of PNP.

References

International Diabetes Federation, Diabetes Atlas, 7th ed., 2015, International Diabetes Federation, Brussels.

L.E. Egede, C. Ellis, Diabetes Research Clinical Practice, 2010, 87, 302.

S.D. Aja, The state of Pet Health 2016 Report, Benfield Pet Hospital's 2016; Section One: 12-14.

C.D. Geddes, J.R. Lakowicz, Reviews in Fluorescence, Springer US., 2006;

I. Sadowska-Bartosz, S. Galiniak, G. Bartosz, Molecules, 2014, 19, 18828.

S.A. Bhat, A. Sohail, A.A. Siddiqui, B. Bano, Journal of Fluorescence, 2014, 24, 1107.

P. Gkogkolou, M. Böhm, Dermatoendocrinological, 2012, 4, 259.

S. Comazzi, W. Bertazzolo, U. Bonfanti, V.S.P. Spagnolo, Research in Veterinary Science, 2008, 84, 341.

A. Senfeld, Z. Pavlíček, Journal of Fluorescence, 1993, 3, 219.

A. Zinellu, S. Sotgia, A.A. Mangoni, M. Sanna, A.E. Satta, C. Carru, Nutrition. Metabolism and Cardiovascular Diseases, 2015, 25,153.

F. Bartoli, G. Carrà, C. Crocamo, D. Carretta, D. La Tegola, T. Tabacchi, P. Gamba, M. Clerici, International Journal of Geriatric Psychiatry, 2016, 31, 829.

R. Herrera, G. Manjarrez, J. Hernandez, Nutrition Neuroscience, 2005, 8, 57.

G.Z. Réus, K. Jansen, S. Titus, A.F. Carvalho, V. Gabbay, J. Quevedo, Journal of Psychiatric Research, 2015, 68, 316.

A. Negre-Salvayre, R. Salvayre, N. Augé, R. Pamplona, M. Portero-Otín, Antioxidants and Redox Signaling, 2009, 11, 3071.

V.P. Singh, A. Bali, N. Singh, J.A. Singh, Korean Journal of Physiology and Pharmacology, 2014, 18, 1.

R. Wada, Y. Nishizawa, N. Yagihashi, M. Takeuchi, Y. Ishikawa, K. Yasumura, M. Nakano, S. Yagihashi, European Journal of Clinical Investigation, 2001, 31, 513.

K Sugimoto, Y Nishizawa, S Horiuchi, S. Yagihashi, Diabetologia, 1997, 40, 1380.

J.W. Baynes, S.R. Thorpe, Diabetes, 1999, 48, 1.

R. Wada, S. Yagihashi, Annals of the New York Academy of Sciences, 2005, 1043, 598.

R. Bucala, Z. Makita, T. Koschinsky, A. Cerami, H. Vlassara, Proceedings of the National Academy of Sciences of the United States of America, 1993, 90, 6434.

J.B. Chang, N.F. Chu, J.T. Syu, A.T. Hsieh, Y.R. Hung, Lipids Health Disease, 2011, 10, 228.

K.A. Polyzos, D.F. Ketelhuth, Hamostaseologie, 2015, 35, 128.

L. Zhang, O. Ovchinnikova, A. Jönsson, A.M. Lundberg, M. Berg, G.K. Hansson, D.F. Ketelhuth, European Heart Journal, 2012, 33, 2025.

M. Brownlee, A. Cerami, H. Vlassara, The New England Journal of Medicine, 1988, 318, 1315.

V. Vishwanath, K.E. Frank, C.A. Elmets, P.J. Dauchot, V.M. Monnier, Diabetes, 1986, 35, 916.

M. Kalousová, J. Skrha, T. Zima, Physiological Research, 2002, 51, 597.

K. Pawlak, T. Domaniewski, M. Mysliwiec, D. Pawlak, Atherosclerosis, 2009, 204, 309.

D. Pawlak, A. Tankiewicz, T. Matys, W. Buczko, Journal of Physiology and Pharmacology, 2003, 54, 175.

D. Pawlak, K. Pawlak, J. Malyszko, M. Mysliwiec, W. Buczko, International Urology and Nephrology, 2001, 33, 399.

M.A. Ahmadou, S.M. Joha, International Journal of Academic Scientific Research, 2015, 3, 101.

M. Hattori, Y. Kotake, Y. Kotake, Acta Vitaminologica et Enzymologica, 1984, 6, 221.

A.D. Patterson, J.A. Bonzo, F. Li, K.W. Krausz, G.S. Eichler, S. Aslam, X. Tigno, J.N. Weinstein, B.C. Hansen, J.R. Idle, F.J. Gonzalez, The Journal of Biological Chemistry, 2011, 286, 19511.

G. Oxenkrug, Molecular Neurobiology, 2013, 48, 294.

Y. Noto, H. Okamoto, Acta Diabetologica Latina, 1978, 15, 273.

A.M. Myint, Y.K. Kim, R. Verkerk, S. Scharpé, H. Steinbusch, B. Leonard, Journal of Affective Disorders, 2007, 98, 143.

D.A. Bender, G.M. McCreanor, Biochemical Society Transactions, 1985, 13, 441.

A. Chiarugi, M. Calvani, E. Meli, E. Traggiai, F. Moroni, Journal of Neuroimmunology, 2001, 120, 190.

A. Akbarzadeh, D. Norouzian, M.R. Mehrabi, S. Jamshidi, A. Farhangi, A.A. Verdi, S.M.A. Mofidian, B.L. Rad, Indian Journal of Clinical Biochemistry, 2007, 22, 60;

Y.S. Kim, N.H. Kim, S.W. Lee, Y.M. Lee, D.S. Jang, J.S. Kim, European Journal of Pharmacology, 2007, 569, 171.

A.D. Association, Classification and diagnosis of diabetes, Diabetes Care, 2015, 38, S8.

G. Münch, R. Keis, A. Wessels, P. Riederer, U. Bahner, A. Heidland, T. Niwa, H.D. Lemke, D. Schinzel, European Journal of Clinical Chemistry and Clinical Biochemistry, 1997, 35, 669.

D.M. Ciobanu, L.E. Olar, R. Stefan, I.A. Veresiu, C.G. Bala, P.A. Mircea, G. Roman, Journal of Diabetes and its Complications, 2015, 29, 893.

W.G. Lesniak, A. Jyoti, M.K. Mishra, N. Louissaint, R. Romero, D.C. Chugani, S. Kannan, R.M. Kannan, Analitical Biochemistry, 2013, 443, 222.

L.E. Olar, R. Stefan, C. Berce, D. Ciobanu, I. Papuc, Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2015, 72, 106.