METABOLIC ENGINEERING OF E. COLI: INFLUENCE OF GENE DELETIONS AND HETEROLOGOUS GENES ON PHYSIOLOGICAL TRAITS

Authors

  • Réka SINKLER Faculty of Applied Chemistry and Materials Science, Polytechnica University, Bucharest, Romania. Corresponding author: miklossyildiko@uni.sapientia.ro.
  • Márta BOTH-FODOR Faculty of Applied Chemistry and Materials Science, Polytechnica University, Bucharest, Romania. Corresponding author: miklossyildiko@uni.sapientia.ro.
  • Emőke ANTAL Faculty of Applied Chemistry and Materials Science, Polytechnica University, Bucharest, Romania. Corresponding author: miklossyildiko@uni.sapientia.ro.
  • Hunor BARTOS Doctoral School of Chemistry, Faculty of Natural Sciences, University of Pécs, Hungary. Corresponding author: miklossyildiko@uni.sapientia.ro.
  • Szabolcs LÁNYI Faculty of Sciences, University of Pécs, Hungary; Department of Bioengineering, Faculty of Economics, Socio-Human Sciences and Engineering, Sapientia Hungarian University of Transylvania, Miercurea Ciuc, Romania. Corresponding author: miklossyildiko@uni.sapientia.ro. https://orcid.org/0000-0003-1592-695X
  • Ildikó MIKLÓSSY Faculty of Sciences, University of Pécs, Hungary; Department of Bioengineering, Faculty of Economics, Socio-Human Sciences and Engineering, Sapientia Hungarian University of Transylvania, Miercurea Ciuc, Romania. Corresponding author: miklossyildiko@uni.sapientia.ro. https://orcid.org/0000-0002-8037-7216

DOI:

https://doi.org/10.24193/subbchem.2019.2.13

Keywords:

1,4-butanediol, heterologous enzyme, Escherichia coli, metabolic engineering

Abstract

1,4-butanediol (BDO) is an important commodity molecule that is used as a platform chemical for the production of polybutylene terephthalate (PBT), elastic fibres (Spandex) and other materials. The homologous enzyme of E. coli, succinyl-CoA synthetase (sucCD) and the heterologous malonyl-CoA reductase from Chloroflexus aurantiacus (mcr) are key enzymes in a heterologous pathway leading to BDO production, which were introduced into a genome-engineered E. coli MG1655(DE3) ΔldhA, ΔpflB strain. Knowing that the expression of recombinant proteins and gene deletions can significantly influence cellular viability, the present study was carried out to investigate the impact of the two key enzyme expression on deletion strains, helping us to analyze the physiological changes of E. coli strains and providing directions for further optimizations in order to achieve satisfying target product yields.

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STUDIA UBB CHEMIA, Volume 64 (LXIV), No.2, Tome I, June 2019

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Published

2019-06-03

How to Cite

SINKLER, R. ., BOTH-FODOR, M. ., ANTAL, E. ., BARTOS, H. ., LÁNYI, S. ., & MIKLÓSSY, I. . (2019). METABOLIC ENGINEERING OF E. COLI: INFLUENCE OF GENE DELETIONS AND HETEROLOGOUS GENES ON PHYSIOLOGICAL TRAITS. Studia Universitatis Babeș-Bolyai Chemia, 64(2), 159–174. https://doi.org/10.24193/subbchem.2019.2.13

Issue

Volume 64, No. 2, Tome I, 2019

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