ISOLATION, PURIFICATION AND CHARACTERIZATION OF ASCORBATE OXIDASE AND PEROXIDASE FROM CUCURBITA PEPO MEDULLOSA
DOI:
https://doi.org/10.24193/subbchem.2019.2.04Keywords:
ascorbate oxidase, peroxidase, field pumpkin, purificationAbstract
Ascorbate oxidase is a well-known copper-containing enzyme however, its biological role and mechanism are still unclear. Reported here is a new protocol for purification of ascorbate oxidase from Cucurbita pepo medullosa (field pumpkin), with improved yields. The enzyme is biochemically characterized, including Michaelis-Menten parameters. Also, purification and characterization of the peroxidase from the same source is described, featuring an unusually high affinity for hydrogen peroxide(low-micromolar).
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