NOVEL POLYDIOXANONE - PLURONIC 127 OXYTOCIN IMPREGNATED MESH, PRELIMINARY STUDY OF OXYTOCIN RELEASE IN VITRO
DOI:
https://doi.org/10.24193/subbchem.2020.4.05Keywords:
polydioxanone, Oxytocin, bone reconstruction, biodegradable scaffolds, RP-HPLC, SEMAbstract
Polymers represent a novel alternative in bone regeneration. Polydioxanone combined with Pluronic was used as a carrier for Oxytocin in form of an interfacial deposition obtained scaffold. The low rate of resorption of the scaffold follow the bone regeneration sequence assuring a constant and continuous release of Oxytocin. The Oxytocin release was monitored by RP-HPLC method. The separation was performed on a Phenomenex Luna 5µm C18 100A, 250x4.6mm, using isocratic elution with acetonitrile: phosphate buffer pH 7 (30:70 v/v) mobile phase. Flow rate 1 ml / min, column temperature 30°C, DAD, l= 220nm. The surface morphology of polydioxanone - pluronic 127 - Oxytocin impregnated mesh was characterized by SEM. The preliminary results show that the Polydioxanone combined with Pluronic is a promising polymer for bone regeneration engineering.
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