ANTITUMOR AND ANTIOXIDANT POTENTIAL OF MAJORANA HORTENSIS EXTRACT BINDING TO THE SILVER NANOPARTICLES ON LUNGS CANCER CELL LINE

Abstract

Majorana hortensis is widely distributed in the Mediterranean area with different medicinal potentials. In the current study, 1 mmol/L AgNO₃ and 4% hydro-methanol leaf extract of M. hortensis at neutral pH (7.0) were successfully combined to create green Majorana hortensis – silver nanoparticles (MHE-AgNPs) after applying the reaction to sunlight for 30 minutes. MHE-AgNPs were characterized using atomic force microscope (AFM), high resolution-scanning electron microscope (HR-SEM) and energy-dispersive X-Ray (EDX). The size range of the MHE-AgNPs was between 50 and 95 nm, and they had a spherical form with smooth surface. With an IC₅₀ of 36.39 µg/mL, MHE-AgNPs exhibited a 2,2′-diphenylpicrylhydrazyl (DPPH) scavenging ability in a concentration-dependent manner. The MTT colorimetric technique was used to determine the MHE-AgNPs cytotoxicity against A549 cell line. The green synthesis of MHE-AgNPs markedly improved the cytotoxic action of MHE-AgNPs against A549 cells. The multiparametric cytotoxicity assay using High-content Screening was employed. MHE-AgNPs was significantly reduced the cell viability, increased the membrane permeability, reduction in mitochondrial potential and stimulating the release of cytochrome c indicating the capability of MHE-AgNPs in killing A549 cells.