ICP-OES AND LC-ESI-MS/MS ANALYSES, ENZYME INHIBITION AND DNA PROTECTION POTENTIAL OF "PELARGONIUM QUERCETORUM" AGNEW

Authors

  • Ebru AKKEMİK Faculty of Engineering, Food Engineering, Siirt University, Kezer Campus, 56100, Siirt, Türkiye; Science and Technology Application and Research Center, Siirt University, Kezer Campus, 56100-Siirt, Türkiye; eakkemik@siirt.edu.tr
  • Mehmet FİDAN Department of Biology, Faculty of Science and Literature, Siirt University, 56100, Siirt, Türkiye. mfidan7384@hotmail.com
  • Merve BALABAN Science and Technology Application and Research Center, Siirt University, Kezer Campus, 56100-Siirt, Türkiye.merve.balaban@siirt.edu.tr
  • Behcet İNAL Department of Agricultural Biotechnology, Faculty of Agriculture, Siirt University, 56100, Siirt, Türkiye. behcetinal@siirt.edu.tr

DOI:

https://doi.org/10.24193/subbchem.2022.4.13

Keywords:

Acetylcholinesterase; Carbonic anhydrase; DNA protection; ICP-OES; P. quercetorum Agnew

Abstract

Pelargonium quercetorum Agnew extract affects activities of acetylcholinesterase (AChE) and carbonic anhydrase I-II enzymes (hCA I-II) was investigated under in vitro conditions. IC50 values of P. quercetorum Agnew on hCA I-II and AChE activity were determined as 0.144±0.0720, 0.209±0.0593, and 0.062±0.0097 mg/mL, respectively. Rutin and shikimic acid was found to be the main phenolic component in P. quercetorum Agnew flower extract from the results of LC-ESI-MS/MS analysis. Rutin was found to be the main phenolic component in P. quercetorum Agnew leaf extract from the results of LC-ESI-MS/MS analysis. ICP-OES analysis showed that the leaves of P. quercetorum Agnew were rich in potassium. The DNA protective effect on plasmid DNA was demonstrated by using extracts obtained from leaf and flower tissues. Consequently, based on the findings of the current study, it could be anticipated that clinical trials related to P. quercetorum Agnew could be completed and the plant could be used pharmacologically.

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STUDIA UBB CHEMIA, Volume 67 (LXVII), No. 4, December 2022

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Published

2022-12-30

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AKKEMİK, E., FİDAN, M., BALABAN, M., & İNAL, B. (2022). ICP-OES AND LC-ESI-MS/MS ANALYSES, ENZYME INHIBITION AND DNA PROTECTION POTENTIAL OF "PELARGONIUM QUERCETORUM" AGNEW. Studia Universitatis Babeș-Bolyai Chemia, 67(4), 197–213. https://doi.org/10.24193/subbchem.2022.4.13

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Volume 67, No. 4, 2022

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