ICP-OES AND LC-ESI-MS/MS ANALYSES, ENZYME INHIBITION AND DNA PROTECTION POTENTIAL OF "PELARGONIUM QUERCETORUM" AGNEW

Authors

  • Ebru AKKEMİK Faculty of Engineering, Food Engineering, Siirt University, Kezer Campus, 56100, Siirt, Türkiye; Science and Technology Application and Research Center, Siirt University, Kezer Campus, 56100-Siirt, Türkiye; eakkemik@siirt.edu.tr
  • Mehmet FİDAN Department of Biology, Faculty of Science and Literature, Siirt University, 56100, Siirt, Türkiye. mfidan7384@hotmail.com
  • Merve BALABAN Science and Technology Application and Research Center, Siirt University, Kezer Campus, 56100-Siirt, Türkiye.merve.balaban@siirt.edu.tr
  • Behcet İNAL Department of Agricultural Biotechnology, Faculty of Agriculture, Siirt University, 56100, Siirt, Türkiye. behcetinal@siirt.edu.tr

DOI:

https://doi.org/10.24193/subbchem.2022.4.13

Keywords:

Acetylcholinesterase; Carbonic anhydrase; DNA protection; ICP-OES; P. quercetorum Agnew

Abstract

Pelargonium quercetorum Agnew extract affects activities of acetylcholinesterase (AChE) and carbonic anhydrase I-II enzymes (hCA I-II) was investigated under in vitro conditions. IC50 values of P. quercetorum Agnew on hCA I-II and AChE activity were determined as 0.144±0.0720, 0.209±0.0593, and 0.062±0.0097 mg/mL, respectively. Rutin and shikimic acid was found to be the main phenolic component in P. quercetorum Agnew flower extract from the results of LC-ESI-MS/MS analysis. Rutin was found to be the main phenolic component in P. quercetorum Agnew leaf extract from the results of LC-ESI-MS/MS analysis. ICP-OES analysis showed that the leaves of P. quercetorum Agnew were rich in potassium. The DNA protective effect on plasmid DNA was demonstrated by using extracts obtained from leaf and flower tissues. Consequently, based on the findings of the current study, it could be anticipated that clinical trials related to P. quercetorum Agnew could be completed and the plant could be used pharmacologically.

References

S. Alp, Bagbahce J. Sci 2017, 4(3), 14-19.

I. Uce, M. Tuncturk, Res. J. Biol. Sci 2014, 7(2), 21-25.

N. Aztopal, B. Cevatemre, M. Sarımahmut, F. Arı, E. Dere, M.Z. Ozel, M. Fırat M, E. Ulukaya, Oncology Letters 2016, 12, 1429-1437.

A. A. Taherpour, K. Kheradmand, As. J. Chem 2008, 20(8), 6335-6341.

G. S. Karatoprak, M. Fırat, M. Kosar, Mersin University J. Health Sci 2018 11(2), 174-183.

F. Arı, S. Celikler, D. Karakas, B. Cevatemre, M. Fırat. E. Ulukaya, J. Clin. Exp. Invest 2017, 8(1), 22-30.

O. I. Kufrevioglu, E. Keha, ‘Biochemistry’, ISBN: 9789758986200, Türkiye, 2018.

O. D. Lopina, in ‘Chapter 11: Enzyme inhibitors and activators’ Intech, 2017, p243-257.

K. A. Askar, A. C. Kudi, A. J. Moody, Can. J. Vet. Res 2011, 75(4), 261–270.

J. Grutzendler, J. C. Morris, Drugs 2001, 61(1), 41-52.

V. Zarotsky, J. J. Sramek, N. R. Cutler, Am. J. Health Syst. Pharm 2003, 60(5), 446–452.

K. Sharma, Mol. Med. Reports 2019, 20, 1479-1487.

D. M. Quinn, Chem. Rev 1987, 87, 955-979.

A. Rahman, M. I. Choudhary, Pure Appl. Chem 2009, 73(3), 555–560.

S. Lee, D. Lee, J. Baek, E. B. Jung, J. Y. Baek, I. K. Lee, T. S. Jang, K. S. Kang, K. H. Kim, Pharm. Biol 2017, 55(1), 2205–2210.

K. Hostettmann, A. Borloz, A. Urbain, A. Marston, Cur. Org. Chem, 2006, 10(8), 825-847.

D. Vullo, S. Del Prete, A. Nocentini, S. M. Osman, Z. Al Othman, C. Capasso, M. Bozdag, F. Carta, P. Gratteri, C. T. Supuran, Bioorg. Med. Chem 2017, 25(3), 1260-1265.

U. M. Kocyigit, Y. Budak, M. G. Gürdere, N. Duru, P. Taslimi, I. Gulcin, M. Ceylan, Monatsh. Chem 2019, 150, 721-731.

M. Boztas, Y. Cetinkaya, M. Topal, I. Gulcin, A. Menzek, E. Sahin, M. Tanc, C. T. Supuran, J. Med. Chem 2015, 58(2), 640-650.

H. Goksu, M. Topal, A. Keskin, M. S. Gultekin, M. Celik, I. Gulcin, M. Tanc, C. T. Supuran, Arch. Pharm 2016, 349(6), 466-74.

E. Akkemik, B. Cicek, Y. Camadan, U. Calisir, Z. Onbasioglu, J. Biochem. Mol. Toxicol 2018, 32(3), e22032.

S. U. Chon, Y. M. Kim, Y. J. Park, B. G. Heo, Y. S. Park, S. Gorinstein, Eur. Food Res. Technol. 2009, 230(2), 231–237.

M. Krasavin, M. Korsakov, Z. Zvonaryova, E. Semyonychev, T. Tuccinardi, S. Kalinin, M. Tanc, C. T. Supuran, Bioorg. Med. Chem 2017, 25(6), 1914-1925.

B. Tepe, S. Degerli, S. Arslan, E. Malatyali, C. Sarikurkcu, Phytoter 2011, 82(2), 237–246.

F. Tao, B. Gonzales-Flecha, L. Kobzik, Free Rad. Biol. Med 2003, 35(4), 327-340.

S. Durdagi, M. Senturk, D. Ekinci, H. T. Balaydin, S. Goksu, O. I. Kufrevioglu, A. Innocenti, A. Scozzafava, C. T. Supuran, Bioorg. Med. Chem 2011, 19(4), 1381-1389.

E. Akkemik, U. Calısır, B. Cicek, J. BAUN Inst. Sci. Technol 2017, 19(2), 192-199.

Y. Camadan, B. Cicek, S. Adem, U Calisir, E. Akkemik, J. Biomol. Struct. Dyn. 2021, 1–10.

M. I. Hassan, B. Shajee, A. Waheed, F. Ahmad, W. S. Sly, Bioorg Med Chem. 2013, 21(6), 1570-1582.

J. Wiesner, Z. Kriz, K. Kuca, D. Jun, J. Koca J, J. Enzyme Inhib. Med Chem. 2007, 22, 417–424.

D. Szwajgier, Annals Agri Env Med. 2015, 22(4), 690–694.

M. Boukhris, M. S. J. Simmonds, S. Sayadi, M. Bouaziz, Phytother. Res 2013, 27(8), 1206-1213.

D. K. Bisset, R. A. Antonia, L. W. B. Browne, J. Fluid Mech. 1990, 218, 439-461.

A. B. Hashkavayi, S. Hashemnia, S. Osfouri, Microchemical J. 2020, 159, 105455.

S. M. Hadi, S. H. Bhat, A. S. Azmi, S. Hanif, U. Shamim, M. F. Ullah, Semin. Cancer Biol 2007 17(5), 370−376.

S. Kumar, S. Sharma, S. K. Chattopadhyay, Fitoterapia 2013, 89, 86-125.

A. S. Yaglıoglu, F. Eser, S. Tekin, A. Onal, Fron. Life Sci 2016, 9(1), 69-74.

Y. Akbaba, E. Bastem, F. Topal, I. Gulcin, A. Maras, S. Goksu, Arch. Pharm 2014, 347, 950–957.

H. Gocer, A. Akıncıoglu, S. Goksu, I. Gulcin, C. T. Supuran, J. Enzyme Inhib. Med. Chem 2015, 30(2), 316–320.

J. Smirnovienė, V. Smirnovas, D. Matulis, Anal Biochem 2017, 522, 61-72.

E. Akkemik, A. Aybek, I. Felek, Appl. Ecol. Environ. Res. 2019, 17(6), 14699-14713.

M. M. Bradford, Anal Biochem 1976, 72, 248-254.

U. K. Laemmli, Nature 1970, 227, 680-685.

J. A. Verpoorte, S. Mehta, J. T. Edsall, J. Biol. Chem 1967, 242(422), 1-9.

G. L. Ellman, K. D. Courtney, V. Andres, R. M. Featherston, Biochem. Pharmacol 1961, 7(2), 88-95.

X. Zhang, P. Feng, X. Gao, B. Wang, C. Gou, R. Bian, Pharm. Biol 2020, 58(1), 247-252.

H. Lineweaver, D. Burk, J.Am. Chem. Soc., 1934, 56, 658.

Ü. Yırtıcı, A. Ergene, M.N. Atalar, & Ş. Adem, South African Journal of Botany, 2022, 144, 58-71.

M.A. Yilmaz, Ind. Crops Prod. 2020, 149, 112347.

http://www.onlinecas.com/Berghof/mWS3/Micronde%20MWS2/applications%20MWS2/AR_MWS-2_Food-Pharma-Cosmetics_140405.pdf, 14.07.20-15:30

K. W. Barnes, E. Debrah, Atomic Spectroscopy 1997, 18, 41-54.

https://www.perkinelmer.com/PDFs/downloads/ATL_BarnesFoodAtomicSpec.pdf‘, 2020b, Accession date: 14.07.20-15:32.

C. Li, L. Chen, J. Chen, Mol. Cell Biol 2002, 22(21), 7562-7571.

STUDIA UBB CHEMIA, Volume 67 (LXVII), No. 4, December 2022

Downloads

Published

2022-12-30

How to Cite

AKKEMİK, E., FİDAN, M., BALABAN, M., & İNAL, B. (2022). ICP-OES AND LC-ESI-MS/MS ANALYSES, ENZYME INHIBITION AND DNA PROTECTION POTENTIAL OF "PELARGONIUM QUERCETORUM" AGNEW. Studia Universitatis Babeș-Bolyai Chemia, 67(4), 197–213. https://doi.org/10.24193/subbchem.2022.4.13

Issue

Volume 67, No. 4, 2022

Section

Articles

License

Copyright (c) 2022 Studia Universitatis Babeș-Bolyai Chemia

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Similar Articles

<< < 3 4 5 6 7 8 9 > >> 

You may also start an advanced similarity search for this article.