Characterization of Some Plant Extracts by Ultrasound-Assisted Extraction in Sunflower Oil Using Thin Layer Chromatography and Spectrophotometry UV-VIS
DOI:
https://doi.org/10.24193/subbchem.2024.2.05Keywords:
sunflower oil, ultrasound-assisted extraction, TLC, UV-VIS, GC-MSAbstract
The purpose of this work was to prove the enrichment of sunflower oil with active principles (polyphenolic acids, flavones, flavonoids) from plants, using the ultrasounds-assisted extraction (UAE) process. The analytical technique used for qualitative characterization of the UAE samples is thin layer chromatography (TLC), a relatively simple technique, which under certain separation and detection conditions highlights the bioactive components of the plants studied in our study. UV-VIS spectrophotometry was used to evaluate the level of flavonoids. The UAE samples in sunflower oil were also characterized by determining the alpha-tocopherol content through a gas-cromatographic method (GC-MS), and by evaluating the antioxidant activity through an indirect DPPH spectrophotometric method. The oily plants extracts were obtained to be used as raw material in the formulation of a dermato-cosmetic product, and thus the characterisation performed herein is very useful in supporting the effects of the above-mentioned products.
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