CHEMICAL COMPOSITION, ANTIMICROBIAL AND MOSQUITO LARVICIDAL ACTIVITIES OF THE ESSENTIAL OIL OF CHLORANTHUS ERECTUS COLLECTED IN VIETNAM
DOI:
https://doi.org/10.24193/subbchem.2026.1.12Keywords:
Chloranthus erectus, essential oil, antimicrobial activity, Candida albicans, mosquito larvicidal activityAbstract
Chloranthus erectus, a subshrub native to South and Southeast Asia, has traditionally been used for its medicinal properties. This study investigated the chemical composition of the essential oil extracted from the aerial parts of C. erectus collected in Vietnam and evaluated its antimicrobial and mosquito larvicidal activities. The essential oil was obtained by hydrodistillation with a yield of 0.21% (v/w) and analyzed using gas chromatography coupled with flame ionization detection and mass spectrometry (GC–FID/MS). The major constituents identified were (E)-β-ocimene (13.41%), myrcene (12.85%), spathulenol (12.55%), and bicyclogermacrene (12.01%). Antimicrobial activity was assessed by determining the minimum inhibitory concentration (MIC) and half-maximal inhibitory concentration (IC50) against selected bacterial and fungal strains. The essential oil showed antimicrobial activity, particularly against Candida albicans (MIC: 16 µg/mL; IC50: 8.96 µg/mL), as well as against Gram-positive bacteria, including Bacillus cereus and Enterococcus faecalis. Larvicidal assays demonstrated toxicity against larvae of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus, with lethal concentration (LC50 and LC90) values below 100 µg/mL after 24 and 48 h of exposure. These results highlight the bioactive potential of C. erectus essential oil for pharmaceutical and environmentally friendly mosquito control applications.
References
1. Y.Y. Liu; Y.Z. Li; S.Q. Huang; H.W. Zhang; C. Deng; X.M. Song; D.D. Zhang; W. Wang; Arab. J. Chem., 2022, 15(11), 104260. https://doi.org/10.1016/j.arabjc.2022.104260
2. A.R. Wang; H.C. Song; H.M. An; Q. Huang; X. Luo; J.Y. Dong; Chem. Biodivers., 2015, 12(4), 451-473. https://doi.org/10.1002/cbdv.201300376
3. Y.J. Xu; Chem. Biodivers., 2013, 10(10), 1754-1773. https://doi.org/10.1002/cbdv.201200066
4. H. Tag; N.D. Namsa; A.K. Das; P. Kalita; S.C. Mandal; J. Ethnopharmacol., 2009, 126(2), 371-374. https://doi.org/10.1016/j.jep.2009.08.015
5. N.A. Hasan; S. Ariffin; A.M. Azzeme; N.I. Hasbullah; M.Z. Nawahwi; I.H.B. Zemry; Mater. Today: Proc., 2023, 88(2), 6-9. https://doi.org/10.1016/j.matpr.2023.01.365
6. H. Tag; N.D. Namsa; M. Mandal; P. Kalita; A.K. Das; S.C. Mandal; Indian J. Pharmacol., 2010, 42(5), 273-276. https://doi.org/10.4103/0253-7613.70083
7. A.F. Musayyaf; International Journal of Medical Science and Clinical Research Studies, 2024, 4(12), 2418-2422. https://doi.org/10.36490/journal-jps.com.v8i3.950
8. I.H. Zemry; N.A. Hasan; N.I. Hasbullah; M.Z. Nawahwi; A.M. Azzeme; D.A. Siti Noor; S. Ariffin; J. Exp. Biol. Agric. Sci., 2023, 11(1), 75-80. http://dx.doi.org/10.18006/2023.11(1).75.80
9. F. Bakkali; S. Averbeck; D. Averbeck; M. Idaomar; Food Chem. Toxicol., 2008, 46(2), 446-475. https://doi.org/10.1016/j.fct.2007.09.106
10. J.F. Kang; Y. Zhang; Y.L. Du; Z.Z. Wang; Z. Naturforsch. C, 2010, 65(11-12), 660-666. https://doi.org/10.1515/znc-2010-11-1205
11. J. Zhu; R. Jia; P. Lai; Chem. Nat. Compd., 2017, 53, 159-161. https://doi.org/10.1007/s10600-017-1936-0
12. H. Tesso; W.A. König; P.T. Son; P.M. Giang; Flavour Fragr. J., 2006, 21(4), 592-597. https://doi.org/10.1002/ffj.1528
13. T.D. Thang; D.N. Dai; I.A. Ogunwande; Chem. Nat. Compd., 2016, 52, 149-151. https://doi.org/10.1007/s10600-016-1575-x
14. L. Chen; Y.M. Huang; M.Y. Sun; M. Zhao; Y.X. Wu; Z.B. Wan; X. Su; C.H. Zhao; J. Northeast For. Univ., 2021, 49(12), 52-57. https://doi.org/10.13759/j.cnki.dlxb.2021.12.021
15. J. Mailina; W.A.W.M. Azrul; M.A.N. Azah; S.S. Husni; J.A. Majid; L. Sahrim; Z.P.M. Faridz; C.C.K. Richard; A.L. Nurliyana; A. Noorsiha; Volatile composition and antifungal properties of Chloranthus erectus leaves oil, in Proceedings of the 15th Seminar on Medicinal and Aromatic Plants, Malaysia, 2018.
16. A.C. Figueiredo; J.G. Barroso; L.G. Pedro; J.J. Scheffer; Flavour Fragr. J., 2008, 23(4), 213-226. https://doi.org/10.1002/ffj.1875
17. A. Barra; Nat. Prod. Commun., 2009, 4(8), 1147-1154. https://doi.org/10.1177/1934578X0900400827
18. L. Serwecińska; Water, 2020, 12(12), 3313. https://doi.org/10.3390/w12123313
19. Y. Zhang; M. Wang; M. Huang; J. Zhao; Front. Microbiol., 2024, 15, 1488106. https://doi.org/10.3389/fmicb.2024.1488106
20. B.F.M.T. Andrade; L.N. Barbosa; I.D.S. Probst; A.F. Júnior; J. Essent. Oil Res., 2014, 26(1), 34-40. https://doi.org/10.1080/10412905.2013.860409
21. S. Chouhan; K. Sharma; S. Guleria; Medicines, 2017, 4(3), 58. https://doi.org/10.3390/medicines4030058
22. R. Pavela; Ind. Crops Prod., 2015, 76, 174-187. https://doi.org/10.1016/j.indcrop.2015.06.050
23. J. Gershenzon; R.B. Croteau; Terpenoid biosynthesis: the basic pathway and formation of monoterpenes, sesquiterpenes, and diterpenes, in Lipid Metabolism in Plants, CRC press, 2018, pp. 339-388.
24. A. Koziol; A. Stryjewska; T. Librowski; K. Salat; M. Gawel; A. Moniczewski; S. Lochynski; Mini-Rev. Med. Chem., 2014, 14(14), 1156-1168. https://doi.org/10.2174/1389557514666141127145820
25. N.A. Mahizan; S.K. Yang; C.L. Moo; A.A.L. Song; C.M. Chong; C.W. Chong; A. Abushelaibi; S.H.E. Lim; K.S. Lai; Molecules, 2019, 24(14), 2631. https://doi.org/10.3390/molecules24142631
26. K.A. Wojtunik‐Kulesza; K. Kasprzak; T. Oniszczuk; A. Oniszczuk; Chem. Biodivers., 2019, 16(12), e1900434. https://doi.org/10.1002/cbdv.201900434
27. H.Y. Li; W.Q. Yang; X.Z. Zhou; F. Shao; T. Shen; H.Y. Guan; J. Zheng; L.M. Zhang; Biomolecules, 2022, 12(9), 1271. https://doi.org/10.3390/biom12091271
28. M. Angane; S. Swift; K. Huang; C.A. Butts; S.Y. Quek; Foods, 2022, 11(3), 464. https://doi.org/10.3390/foods11030464
29. M. Donati; A. Mondin; Z. Chen; F.M. Miranda; B.B. do Nascimento Jr; G. Schirato; P. Pastore; G. Froldi; Nat. Prod. Res., 2015, 29(10), 939-946. https://doi.org/10.1080/14786419.2014.964709
30. O.A. Oyedeji; A.J. Afolayan; J.N. Eloff; S. Afr. J. Bot., 2005, 71(1), 114-116. https://doi.org/10.1016/S0254-6299(15)30160-5
31. I.R. da Silva; C.C. Fernandes; D.S. Gonçalves; C.H. Martins; M.L. Miranda; Nat. Prod. Res., 2024, 38(19), 3476-3480. https://doi.org/10.1080/14786419.2023.2249584
32. A. Shafaghat; M. Shafaghatlonbar; Nat. Prod. Commun., 2011, 6(2), 1934578X1100600230. https://doi.org/10.1177/1934578X1100600230
33. I.H.N. Bassolé; H.R. Juliani; Molecules, 2012, 17(4), 3989-4006. https://doi.org/10.3390/molecules17043989
34. F. Nazzaro; F. Fratianni; L. De Martino; R. Coppola; V. De Feo; Pharmaceuticals, 2013, 6(12): 1451-1474. https://doi.org/10.3390/ph6121451
35. O. Lüderitz; M.A. Freudenberg; C. Galanos; V. Lehmann; E.T. Rietschel; D.H. Shaw; Lipopolysaccharides of gram-negative bacteria, in Current topics in membranes and transport, Academic Press, 1982, pp. 79-151. https://doi.org/10.1016/S0070-2161(08)60309-3
36. M. Gupta; D. Gupta; J. Drug Deliv. Ther., 2022, 12(3), 193-201. http://dx.doi.org/10.22270/jddt.v12i3.5313
37. V.V. Khoa; D.N. Dai; L.T. Huong; B.B. Thinh; Nat. Prod. Commun., 2024, 19(11), 1934578X241299995. https://doi.org/10.1177/1934578X241299995
38. D.N. Dai; N.T. Thao; L.T. Huong; N.H. Hung; V.T. Thuong; B.B. Thinh; J. Essent. Oil Bear. Plants, 2024, 27(5), 1334-1346. https://doi.org/10.1080/0972060X.2024.2414866
39. L.T. Huong; D.N. Dai; N.H. Hung; B.B. Thinh; Chem. Biodivers., 2026, 23(2), e01348. https://doi.org/10.1002/cbdv.202501348
40. J.M.S. de Sousa; T.A.L. Nunes; R.R.L. Rodrigues; J.P.A. de Sousa; M.C.A. Val; F.A.R. Coelho; A.L.S. dos Santos; N.B. Maciel; V.M.R. de Souza; Y.A.A. Machado; P.S.A. Sousa; A.R. de Araújo; J.A. Rocha; D.P. de Sousa; M.V. da Silva; D.D.R. Arcanjo; K.A. da Franca Rodrigues; Pharmaceuticals, 2023, 16(2), 183. https://doi.org/10.3390/ph16020183
41. X. Bai; J. Tang; Nat. Prod. Commun., 2020, 15(9), 1934578X20961189. https://doi.org/10.1177/1934578X20961189
42. V.P. de Almeida; S.E.L. Tolouei; M. Minteguiaga; D.S.A. Chaves; G. Heiden; S.I. Khan; J. Trott; M. Wang; E. Dellacassa; V. Raman; P.V. Farago; I.A. Khan; A.G. Junior; J. Manfron; Chem. Biodivers., 2023, 20(10), e202300862. https://doi.org/10.1002/cbdv.202300862
43. S.S. Grecco; E.G.A. Martins; N. Girola; C.R. de Figueiredo; A.L. Matsuo; M.G. Soares; B.C. Bertoldo; P. Sartorelli; J.H.G. Lago; Pharm. Biol., 2015, 53(1), 137-143. https://doi.org/10.3109/13880209.2014.912238
44. J. Calva; M.B. Cuenca; A. León; Á. Benítez; Molecules, 2025, 30(13), 2712. https://doi.org/10.3390/molecules30132712
45. S. Senthil-Nathan; Front. Physiol., 2020, 10, 473482. https://doi.org/10.3389/fphys.2019.01591
46. M. Jankowska; J. Rogalska; J. Wyszkowska; M. Stankiewicz; Molecules, 2017, 23(1), 34. https://doi.org/10.3390/molecules23010034
47. T.R.S.A. Luz; L.S.S. de Mesquita; F.M.M. do Amaral; D.F. Coutinho; Acta Tropica., 2020, 212, 105705. https://doi.org/10.1016/j.actatropica.2020.105705
48. C.N. Dias; D.F.C. Moraes; Parasitol. Res., 2014, 113, 565-592. https://doi.org/10.1007/s00436-013-3687-6
49. Vietnamese Pharmacopoeia; Medical Publishing House, Hanoi, Vietnam, 2009.
50. B.B. Thinh; D.B. Thin; I.A. Ogunwande; Nat. Prod. Commun., 2024, 19(3), 1934578X241239477. https://doi.org/10.1177/1934578X241239477
51. B.B. Thinh; L.D. Chac; J. Essent. Oil Bear. Plants, 2024, 27(2), 574-583. https://doi.org/10.1080/0972060X.2024.2324347
52. D.N. Dai; L.T. Thuy; D.T. Xuyen; N.T. Viet; L.T. Huong; B.B. Thinh; J. Essent. Oil Bear. Plants, 2025, 28(1), 147-157. https://doi.org/10.1080/0972060X.2025.2463480
53. National Institute of Science and Technology; NIST Chemistry Webbook. Data from NIST Standard Reference Database, 2018, p. 69.
54. R.P. Adams; Identification of essential oil components by gas chromatography/ mass spectroscopy. Allured Publishing Corporation, Carol Stream, 2007.
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