Antimicrobial effects produced by gold nanoparticles obtained with extracts of Allium sativum and Allium ursinum
DOI:
https://doi.org/10.24193/subbbiol.2025.1.15Keywords:
Allium sativum, Allium ursinum, antimicrobial, nanoparticlesAbstract
Gold nanoparticles (AuNPs) obtained by green synthesis using plant extracts from the genus Allium have attracted significant scientific interest due to their potential applications as antimicrobial agents in the biomedical field. This study investigates the antimicrobial potential of AuNPs obtained by green synthesis using extracts of Allium sativum and Allium ursinum. These plant extracts are rich in sulfur compounds (allicin), flavonoids and polyphenols, which not only facilitate the formation of nanoparticles, but also confer them increased antimicrobial properties. The nanoparticles thus obtained were characterized by spectroscopic methods (UV-Vis) and were tested for antimicrobial activity. Microbiological tests performed in vitro demonstrated an antimicrobial activity of the nanoparticles against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria but also against Candida albicans and Candida parapsilosis. The results support the idea that gold nanoparticles functionalized with Allium extracts may constitute a promising alternative in the development of natural antimicrobial compounds with applications in medicine, the food industry and the pharmaceutical field.
Article history: Received 16 May 2025; Revised 29 May 2025;
Accepted 29 May 2025; Available online 25 June 2025
References
Abdelsattar, A.S., Kamel, A.G., Eita, M.A., Elbermawy, Y., & El-Shibiny, A. (2024). The cytotoxic potency of green synthesis of zinc oxide nanoparticles (ZnO-NPs) using Origanum majorana. Mat Lett,. 367, 136654. https://doi.org/10.1016/j.matlet.2024.136654
Baran, M.F., Keskin, C., Baran, A., Hatipoğlu, A., Yildiztekin, M., Küçükaydin, S., Kurt, K.; Hoşgören, H.; Sarker, M.M.R.; Sufianov, A., Beylerli, O., Khalilov, R., & Eftekhari, A. (2023). Green Synthesis of Silver Nanoparticles from Allium cepa L. Peel Extract, Their Antioxidant, Antipathogenic, and Anticholinesterase Activity. Molecules, 28, 2310. https://doi.org/10.3390/molecules28052310
Barbu, I. A., Ciorîță, A., Carpa, R., Moț, A. C., Butiuc-Keul, A., & Pârvu, M. (2023). Phytochemical Characterization and Antimicrobial Activity of Several Allium Extracts. Molecules, 28 (10): 3980. https://doi.org/10.3390/molecules28103980
Bastús, N. G., Comenge, J., & Puntes, V. (2011). Kinetically Controlled Seeded Growth Synthesis of Citrate-Stabilized Gold Nanoparticles of up to 200 nm: Size Focusing versus Ostwald Ripening. Langmuir J, 27 (17): 11098–105. https://doi.org/10.1021/la201938u
Bogale, B.L., Adamu, T.B., Kebede, M.A., Ayana, M.T., Kebede, W.W., & Fetene T. (2025). Green and facile synthesis of silver nanoparticles (Ag NPs) using Rhamnus prinoides (Gesho) leaf extract for antibacterial, antioxidant and photocatalytic activities. Next Nanotechnology, 7: 100163. https://doi.org/10.1016/j.nxnano.2025.100163
Coman, C., Leopold, L. F., Rugină, O. D., Barbu-Tudoran, L., Leopold, N., Tofană, M., & Socaciu, C. (2013). Green Synthesis of Gold Nanoparticles by Allium sativum Extract and Their Assessment as SERS Substrate. J Nanopart Res, 16 (1): 2158. https://doi.org/10.1007/s11051-013-2158-4
Chowdhury, M.A.S., Islam, M.M., Jamal, M. (2025). Green synthesis of nickel oxide nanoparticles using Allium cepa stalks and investigation of their antibacterial activity. Results Chem, 16, 102328. https://doi.org/10.1016/j.rechem.2025.102328
Cui, Z., Hu, J., Jiang, X., Zhang, D., Fang, C. (2021). Asymmetric Au/(PdAg alloy) nano-allium giganteums for their enhanced electrocatalytic performances to ethanol oxidation reaction. J. Alloys Comp, 855, 2, 157385. https://doi.org/10.1016/j.jallcom.2020.157385
Dauthal, P., & Mausumi, M. (2016). Noble Metal Nanoparticles: Plant-Mediated Synthesis, Mechanistic Aspects of Synthesis, and Applications. Ind Eng Chem Res, 55 (36): 9557–77. https://doi.org/10.1021/acs.iecr.6b00861
El-Gebalya, A.S., Sofya, A.R., Hmeda, A.A., Youssef, A.M. (2024). Green synthesis, characterization and medicinal uses of silver nanoparticles (Ag-NPs), copper nanoparticles (Cu-NPs) and zinc oxide nanoparticles (ZnO-NPs) and their mechanism of action: A review. Biocatal Agric Biotech, 55, 103006. https://doi.org/10.1016/j.bcab.2023.103006
Ezhuthupurakkal, P.B., Polaki, L.R., Suyavaran, A., Subastri, A., Sujatha, V., Thirunavukkarasu, C. (2017). Selenium nanoparticles synthesized in aqueous extract of Allium sativum perturbs the structural integrity of Calf thymus DNA through intercalation and groove binding. Mat Sc Eng C, 74, 597–608. http://dx.doi.org/10.1016/j.msec.2017.02.003
Franci, G., Falanga, A., Galdiero, S., Palomba, L., Rai, M., Morelli, G., & Galdiero, M. (2015). Silver Nanoparticles as Potential Antibacterial Agents. Molecules, 20 (5): 8856–74. https://doi.org/10.3390/molecules20058856
Huang, X., & El-Sayed. M. A. (2010). Gold nanoparticles: Optical properties and implementations in cancer diagnosis and photothermal therapy. J Adv Res, 1 (1): 13–28. https://doi.org/10.1016/j.jare.2010.02.002
Jain, P. K., Huang, X., El-Sayed, I.H., & El-Sayed, M. A., (2007). Review of Some Interesting Surface Plasmon Resonance-Enhanced Properties of Noble Metal Nanoparticles and Their Applications to Biosystems. Plasmonics, 2 (3): 107–18. https://doi.org/10.1007/s11468-007-9031-1
Link, S., & El-Sayed, M. A. (2000). Shape and size dependence of radiative, non-radiative and photothermal properties of gold nanocrystals. Int Rev Phy Chem, 19 (3): 409–53. https://doi.org/10.1080/01442350050034180
Ma, W., Zhu, G., Zhang, Y., & Guo, J. (2024). Green synthesis of ZnO NPs with long-lasting and ultra-high antimicrobial activity. Surfaces and Interfaces, 50, 104506. https://doi.org/10.1016/j.surfin.2024.104506
Rabiee, N., Mojtaba Bagherzadeh, Mahsa Kiani, Amir Mohammad Ghadiri, Kaiqiang Zhang, Zhong Jin, Seeram Ramakrishna, & Mohammadreza Shokouhimehr. (2020). High Gravity-Assisted Green Synthesis of ZnO Nanoparticles via Allium ursinum: Conjoining Nanochemistry to Neuroscience. Nano Exp, 1 (2): 020025. https://doi.org/10.1088/2632-959X/abac4d
Saeed, Z., Pervaiz, M., Ejaz, A., Hussain, S., Shaheen, S., Shehzad, B., & Younas, U. (2023). Garlic and ginger extracts mediated green synthesis of silver and gold nanoparticles: A review on recent advancements and prospective applications. Biocatal Agric Biotech, 53, 102868. https://doi.org/10.1016/j.bcab.2023.102868
Sharma, Virender K., Ria A. Yngard, & Yekaterina Lin. (2009). Silver Nanoparticles: Green Synthesis and Their Antimicrobial Activities. Adv Colloid Interface Sc, 145 (1–2): 83–96. https://doi.org/10.1016/j.cis.2008.09.002
Sondi, I., & Branka S.-S. (2004). Silver Nanoparticles as Antimicrobial Agent: A Case Study on E. coli as a Model for Gram-Negative Bacteria. J Colloid Interface Sc, 275 (1): 177–82. https://doi.org/10.1016/j.jcis.2004.02.012
Suleria, H. A. R., Masood, S.B., Nauman, K., Saira, S., Ali, R., Muhammad, A., & Munawar, A. (2015). Garlic (Allium sativum): Diet Based Therapy of 21st Century–a Review, Asian Pac J Trop Dis, 5 (4): 271-278 https://doi.org/10.1016/S2222-1808(14)60782-9
Tan, G., Tevlek, A., & Aydin, H. M.. (2023). Comparison of garlic and onion extract-derived gold nanoparticles: Characterization and anticancer activity. J Drug Deliv Sc Tech, 84, 104542. https://doi.org/10.1016/j.jddst.2023.104542
Uddin, S., Safdar, L. B., Anwar, S., Iqbal, J., Laila, S., Abbasi, B. A., Saif, M. S., Ali, M., Rehman, A., Basit, A., Wang, Y., & Quraishi, U. M. (2021). Green Synthesis of Nickel Oxide Nanoparticles from Berberis balochistanica Stem for Investigating Bioactivities. Molecules, 26(6), 1548. https://doi.org/10.3390/molecules26061548
Yulizar, Y., Ariyanta, H. A., & Abduracman, L. (2017). Green Synthesis of Gold Nanoparticles Using Aqueous Garlic (Allium sativum L.) Extract, and Its Interaction Study with Melamine. Bull Chem React Eng Catal, 12 (2): 212–18. https://doi.org/10.9767/bcrec.12.2.770.212-218
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