ANTIOXIDANT, ANTI-HAEMOLYTIC ACTIVITY, VITAMIN AND ELEMENT PROFILE OF RHAMNUS CORNIFOLIUS

Abstract

The antioxidant, antiradical, and anti-hemolytic activities of the methanol extract of Rhamnus cornifolius leaves were investigated, along with the determination of element and vitamin levels in the dried plant material. Element analyses were determined by ICP-OES dry ashing, vitamins by HPLC, vitamin C, total phenolic, flavonoid, antioxidant capacity, DPPH^•, ABTS^•+ and anti-haemolytic activity spectrophotometrically. Total phenolic, flavonoid and antioxidant capacities of the plant were measured as 4.482 ± 0.159 mg GA/g, 27.420 ± 2.551 mg QE/g and 41.795 ± 18.506 mM AA/g, while retinol, α-tocopherol, phylloquinone and ascorbic acid as 0.2167 ± 0.019, 4.313 ± 0.685, 0.5622 ± 0.09 µmol/kg and 403.631 ± 8.682 mg 100/g. IC₅₀ values of DPPH^•, ABTS^•+ and anti-haemolytic assays were determined as 33.974 ± 1.918 (BHT = IC₅₀ 26.979 ± 1.116), 58.197 ± 1.826 (trolox = IC₅₀ 27.854 ± 1.352) and 68.51 ± 5.33 (trolox = IC₅₀ 44.040 ± 0.290) μg/mL, respectively. According to the results of this study, it was determined that the vitamin and element content of the plant was at high level, ABTS^•+ and DPPH^• tests showed a promising antioxidant power.  These findings represent the first report in the literature, and the plant’s composition was evaluated as a basis for future in vivo studies.