ANALYTICAL STUDY OF GALLSTONES IN PATIENTS FROM TRANSYLVANIA, ROMANIA

Authors

  • Ioana Andreea BREZEȘTEAN Department of Biomolecular Physics, Faculty of Physics, Babes-Bolyai University, Cluj-Napoca, Romania. Email: ioana.brezestean@itim-cj.ro. https://orcid.org/0000-0001-6374-5155
  • Nicolae HAR Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania. Email: nicolae.har@bioge.ubbcluj.ro. https://orcid.org/0000-0002-7332-8969
  • Alina TANȚĂU 4-th Medical Clinic; “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania. Corresponding author: simona.cinta@phys.ubbcluj.ro. https://orcid.org/0000-0001-5975-0779
  • Maria GOREA Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Email: mgorea@chem.ubbcluj.ro.
  • Monica Mihaela VENTER Department of Inorganic Chemistry, Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Email: monica@chem.ubbcluj.ro. https://orcid.org/0000-0002-3149-9831
  • Simona CÎNTĂ PÎNZARU Department of Biomolecular Physics, Babes-Bolyai University, Cluj-Napoca, Romania. Email: simona.cinta@phys.ubbcluj.ro. https://orcid.org/0000-0001-8016-4408

Keywords:

Human gallstones, Transylvania, FT-Raman spectroscopy, FT-IR, cholesterol

Abstract

Here we report the vibrational spectroscopy characterization on human gallstones from patients in Transylvania, Romania. A number of 93 gallstones resulted after surgical intervention were preliminary classified in 6 groups after provenance, shape and color aspect and investigated using optical microscopy and FT-(micro)-Raman spectroscopy in conjunction with the FT-IR. Optical microscopy of the gallstones cross sections revealed concentric layers growing depositions alternating with axial crystalline agglomerated biomaterial. Two distinct type of crystallinity on one hand and different spatial crystals distribution, on the other hand were optically observed. The studied specimens randomly showed very narrow amorphous zones. The microstructure, chemical composition, the nucleation and growing mechanism of gallstones were correlated with the vibrational spectroscopy data. Vibrational assignment allowed identifying cholesterol in all the samples. Other weak bands attributable to bilirubinate salts were randomly observed with different extent in all the groups. Brown, yellowish-brown and yellow gallstones showed higher content of bilirubinate as revealed by the relative intensity ratio of the bilirubinate band at 1620 cm-1 versus cholesterol band at 1464 cm-1 with values between 0.57 and 0.875. FT-vibrational techniques accurately provided the proof of the cholesterol specificity in the choletitiasis prevalence in the area and allowed getting additional insight into their growing mechanism.

References

A.J. Harding, Gallstones: Causes and Treatments. William Heinemann Medical Books, London, 1964.

G.H. Sakorafas, D. Milingos, G. Peros, Digestive Diseases and Science, 2007, 52 (5), 1313

T. Qiao, R-H. Ma, X. Luo, L. Yang, Z. Luo, et al. PLoS ONE, 2013, 8(10), e74887. doi:10.1371/journal.pone.0074887.

Z. Zhang, World Chinese J. Digestol. 2008, 16 (11), 1200.

L.Y. Chen, Q.H. Qiao, S.C. Zhang, Y.H. Chen, G.Q. Chao, et al. World Journal of Gastroenterology, 2012, 18 (31), 4215.

T. Maki, Annals of Surgery, 1966, 164 (1), 90.

G. Elek, A. Rockenbauer, Klinische Wochenschrift 1982,60 (1), 33.

J. Ahlberg, T. Curstedt, K. Einarsson, J. Sjovall, Journal of Lipid Research, 1981, 22 (3) 404.

K. Einarsson, K. Nilsell, B. Leijd, B. Angelin, New England Journal of Medicine, 1985, 313 (5), 277.

C. Paluszkiewicz, W.M. Kwiatek, M. Galka, D. Sobieraj, D. Wentrup-Byrne, Cellular and Molecular Biologu 1998, 44, 65.

B.W.Trotman, R.D.Soloway, 1982, 2:879.

J.J. Roslyn, M.J. Zinner, Principles of Surgery 1994,1376-1399.

M. Donovan, Gastroenterology Clinics of North America 1999, 28 (1), 75.

C.W. Ko, S.P. Lee, Gastroenterology Clinics of North America (1999) 28, 1, 99.

J.G. Wu, G.R. Shen, Y.Y. Gao, X.S. Chou, K.L. Zhang, J.H. Lin, S.G. Zhu, Chinese Scientific Bulletin 1979, 24 (22),1054.

G.D. Offner, D.H. Gong, N.H. Aldhal, Gastroenterology, 1994, 106, 755.

K. Chijiiwa, A. Koga, T. Yamasaki, K. Shimada, H. Noshiro, F. Nakayama, Biochimica Biophysica Acta, 1991, 1086, 44.

P.R.C. Harvey, G.A. Upadhya, S.M. Strasberg, Journal of Biologial Chemistry 1991, 266, 13996.

M. Abei, H. Nuutinen, P. Kawczak, J. Schwarzendrube, S.P. Pillay, R.T. Holzbach, Gastroenterology, 1994, 106, 231.

B. F. Smith, Jouranl of Lipid Research 1987, 28, 1088.

H. Hrbasova, R. Vondruskova, M. Spundova, Z. Marecek, G. Entlicher, International Journal of Biochemistry and Cellular Biology 2000, 32 (6), 609.

G. Liu, Da Xing, Haimin Yang, Jie Wu, J. Mol. Struct. 2002, 616, 187.

M. Acalovschi, D. Blendea, C. Feier, A.I Letia, N. Ratiu, D.L Dumitrascu and A. Veres, American Journal of Gastroenterology 2003, 98, 1856.

M. Tantau, V. Mercea, D. Crisan, A. Tantau,, G. Mester, S. Vesa, Z. Sparchez, Journal of Gastrointestinal Liver Diseases, 2013, 22, 2, 141.

J.R. Ferraro, J.G. Wu, R.D. Soloway, W.H. Li, Y.Z. Xu, D.F.Xu, G.R. Shen, Applied Spectroscopy 1996, 50, 7922.

S. Kothai, K. Gayathri, V. Kannappan, P.T. Perumal, K. Manimegalai, International Journal of Chemical Technological Research, 2009, 1, 3, 430.

B. Yang, R.C. Taylor, M.D. Morri, X.-Z. Wang J.-Guang WV, B.-Z. Yu and G.-X. Xv, Spectrochimica Acta A, 1993, 49, 12., 1735.

W.-H. Li, G.-R. Shen, R. D. Soloway, Z.-L. Yang, X.-B.Tong, E. Wu, D.-F. Xu, J.G. Wu, and G.X. Xu, Biospectroscopy, 1995, 1, 149.

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Published

2015-03-30

How to Cite

BREZEȘTEAN, I. A. ., HAR, N. ., TANȚĂU, A. ., GOREA, M. ., VENTER, M. M. ., & CÎNTĂ PÎNZARU, S. . (2015). ANALYTICAL STUDY OF GALLSTONES IN PATIENTS FROM TRANSYLVANIA, ROMANIA. Studia Universitatis Babeș-Bolyai Chemia, 60(1), 29–43. Retrieved from https://studia.reviste.ubbcluj.ro/index.php/chemia/article/view/8401

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