PROBING REDUCING POWER FOR FERRYL PHYTOGLOBINS OF SEVERAL PHENOLIC COMPOUNDS USING THEIR KINETIC PROFILES ASSISTED BY CHEMOMETRIC METHODS
DOI:
https://doi.org/10.24193/subbchem.2017.2.04Keywords:
phenolics, flavonoids, phytoglobin, reducing powerAbstract
Several phenolic compounds belonging to different classes were comparatively analyzed for their ability to reduce ferryl forms of three non-symbiotic phytoglobins which were generated in situ by hydrogen peroxide and thus acting as enzymes. The kinetic profiles of the substrates oxidation were evaluated using principal component analysis and cluster analysis. The three globins were different not only in terms of rate but also in mechanism and the electron donor ability of the studied phenolics were strongly enzyme specific and did not depend only upon their chemical structure but also upon assumptive binding pocket environment.
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