CHARACTERIZATION OF ORTHOSIPHON STAMINEUS BENTH EXTRACTS BY REVERSED-PHASE THIN LAYER CHROMATOGRAPHIC METHODS

Neli-Kinga  OLAH; Simona Codruța Aurora  COBZAC; Andreea  BRASOVAN; Elisabeta  CHIȘE; Claudiu  MORGOVAN; Claudia-Crina  TOMA; Radu  OPREAN; Ede  BODOKI; Daniela  HANGANU; †Simion  GOCAN

doi:10.24193/subbchem.2017.3.01

CHARACTERIZATION OF ORTHOSIPHON STAMINEUS BENTH EXTRACTS BY REVERSED-PHASE THIN LAYER CHROMATOGRAPHIC METHODS

Authors

Neli-Kinga OLAH Faculty of Pharmacy, Vasile Goldiș Western University, Arad; SC PlantExtrakt SRL, Rădaia, Cluj, Romania. Email: olah.neli@uvvg.ro. https://orcid.org/0000-0001-5204-6885
Simona Codruța Aurora COBZAC Faculty of Chemistry and Chemical Engineering; Research Center for Advanced Chemical Analysis, Instrumentation and Chemometrics – ANALYTICA, Babeş-Bolyai University, Cluj-Napoca, Romania. Email: csimona@chem.ubbcluj.ro.
Andreea BRASOVAN Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Email: tbrasovan@yahoo.com..
Elisabeta CHIȘE Faculty of Pharmacy, Vasile Goldiș Western University, Arad, Romania. Email: c_elisabeta@yahoo.com.
Claudiu MORGOVAN Department of Pharmaceutical Sciences, Faculty of Pharmacy, Vasile Goldis Western University, Arad, Romania. Email: claudiumorgovan@yahoo.com. https://orcid.org/0000-0003-2730-8729
Claudia-Crina TOMA Faculty of Pharmacy, Vasile Goldiș Western University, Arad, Romania. Email: claudiatoma2004@yahoo.com. https://orcid.org/0000-0003-1515-3256
Radu OPREAN Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania. Email: roprean@umfcluj.ro. https://orcid.org/0000-0001-7708-3367
Ede BODOKI Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania. Email: dhanganu@umfcluj.ro. https://orcid.org/0000-0003-4667-2286
Daniela HANGANU Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania. Email: dhanganu@umfcluj.ro. https://orcid.org/0000-0002-5407-8001
†Simion GOCAN Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Corresponding author: csimona@chem.ubbcluj.ro.

DOI:

https://doi.org/10.24193/subbchem.2017.3.01

Keywords:

RP-TLC; RP-AMD; Orthosiphon stamineus Benth. extracts; rosmarinic acid; sinesetine; eupatorine

Abstract

TLC is a powerful method used for separation of complex mixtures such as plant extracts. Employing different TLC techniques the separations can be improved. This paper presents a study of the Orthosiphon stamineus Benth extracts using isocratic reversed-phase thin layer chromatography (RP-TLC) and reversed-phase automated multiple development technique (RP-AMD). Methanol (SI) and a mixture of methanol–water–methyl acetate (SII) were used as extraction agents. Orthosiphon stamineus Benth. belongs to the Lamiaceae family. Its leaves contain rosmarinic acid, sinesetine and eupatorine as main compounds. After TLC separation the bioactive compounds from plant extracts were identified by comparison of the R_f values and in situ UV-Vis spectra with those of the standards and quantified using the calibration method. The rosmarinic acid was better extracted in the solvent mixture methanol–water–methyl acetate (10:10:80, v/v), while the sinesetine and eupatorine, which are more lipophilic, were better extracted in methanol. The study revealed the AMD technique superiority in comparison with the isocratic one.

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STUDIA UBB CHEMIA, Volume 62 (LXII), No. 3, September 2017

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Published

2017-09-29

How to Cite

OLAH, N.-K. ., COBZAC, S. C. A. ., BRASOVAN, A. ., CHIȘE, E. ., MORGOVAN, C. ., TOMA, C.-C. ., … GOCAN, †Simion . (2017). CHARACTERIZATION OF ORTHOSIPHON STAMINEUS BENTH EXTRACTS BY REVERSED-PHASE THIN LAYER CHROMATOGRAPHIC METHODS. Studia Universitatis Babeș-Bolyai Chemia, 62(3), 9–18. https://doi.org/10.24193/subbchem.2017.3.01