PERIODATE-OXIDIZED ALGINATE AS POLYCONDENSATION REAGENT FOR HEMOGLOBIN

Florina  SCURTU; Anamaria  POPA; Radu  SILAGHI-DUMITRESCU

doi:10.24193/subbchem.2017.4.05

PERIODATE-OXIDIZED ALGINATE AS POLYCONDENSATION REAGENT FOR HEMOGLOBIN

Authors

Florina SCURTU Department of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Email: florinadeac@chem.ubbcluj.ro. https://orcid.org/0000-0002-2406-9163
Anamaria POPA Department of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Corresponding author: florinadeac@chem.ubbcluj.ro.
Radu SILAGHI-DUMITRESCU Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca; Romanian Chemical Society, Romanian Society of Biochemistry and Molecular Biology, Society of Biological Inorganic Chemistry, Romania. Email: radu.silaghi@ubbcluj.ro. https://orcid.org/0000-0003-3038-7747

DOI:

https://doi.org/10.24193/subbchem.2017.4.05

Keywords:

blood substitute, hemoglobin, alginate, oxidative stress

Abstract

We have previously demonstrated that derivatization of hemoglobin with periodate-modified sugar derivatives incurs increases in the pro-oxidant reactivity and, also, that serum bovine serum albumin can resolve this problem entirely. Here, we described a new polymer based on hemoglobin and another oxygen-containing compound, alginate. The rate of autooxidation increases after derivatization, but serum albumin alleviates this problem. The peroxide reactivity and oxygen affinity were also tested but no significant differences were observed between derivatized and native hemoglobin.

References

H. Bunn, J.H. Jandl, Journal of Experimental Medicine, 1969, 129, 925

X. Liu, M. J.S. Miller, M.S. Joshi, H. Sadowska-Krowicka, D.A. Clark, J.R. Lancaster, Journal of Biological Chemistry, 1998, 273, 18709

J.R. Hess, R.F. Reiss, Translational Medicine Reviews, 1996, 10, 276

A.I. Alayash, Nature Reviews in Drug Discovery, 2004, 3, 152

D.H. Doherty, M.P. Doyle, S.R. Curry, R.J. Vali, T.J. Fattor, J.S. Olson, D.D. Lemon, Nature Biotechnology, 1998, 16, 672

R.M. Winslow, Artificial Organs, 2004, 28, 800

B.J. Reeder, M. Grey, R.L. Silaghi-Dumitrescu, D.A. Svistunenko, L. Bulow, C.E. Cooper, M.T. Wilson, Journal of Biological Chemistry, 2008, 283, 30780

I. Portoro, L. Kocsis, P. Herman, D. Caccia, M. Perrella, L. Ronda, S. Bruno, S. Bettati, C. Micalella, A. Mozzarelli, A. Varga, M. Vas, K.C. Lowe, A. Eke, Biochimica Biophysica Acta, 2008, 1784, 1402

J.G. Riess, Chemical Reviews, 2001, 101, 2797

F. Deac, A. Todea, R. Silaghi-Dumitrescu Glutaraldehyde derivatization of hemoglobin: a potential blood substitute; Silaghi-Dumitrescu, R. and Garban, G., Ed.; Cluj University Press: Cluj-Napoca, Romania, 2009, pp 165

S.G.R.C., H.H.D., Journal of Biological Chemistry, 1986, 261, 6811

J.H. Eike, A.F. Palmer, Biotechnology Progress, 2004, 20, 953

F. Deac, B. Iacob, E. Fischer-Fodor, G. Damian, R. Silaghi-Dumitrescu, Journal of Biochemistry, 2011, 149, 75

B.J. Reeder, D.A. Svistunenko, C.E. Cooper, M.T. Wilson, Antioxidants& Redox Signaling, 2004, 6, 954

R. Silaghi-Dumitrescu, B.J. Reeder, P. Nicholls, C.E. Cooper, M.T. Wilson, Biochemical Journal, 2007, 403, 391

A.C. Mot, A. Roman, I. Lupan, D.M. Kurtz, R. Silaghi-Dumitrescu, Protein Journal, 2010, 29, 387

M. Arkosi, F. Scurtu, A. Vulpoi, R. Silaghi-Dumitrescu, D.M. Kurtz, Jr., Artificial Cells and Blood Substitutes, 2016, 45, 218

D. Hathazi, A.C. Mot, A. Vaida, F. Scurtu, I. Lupan, E. Fischer-Fodor, G. Damian, D.M. Kurtz, Jr., R. Silaghi-Dumitrescu, Biomacromolecules, 2014, 15, 1920

F. Scurtu, O. Zolog, B. Iacob, R. Silaghi-Dumitrescu, Artificial Cells, Nanomedicine and Biotechnology, 2014, 42, 13

V.F. Scurtu, A.C. Mot, R. Silaghi-Dumitrescu, Pharmaceuticals, 2013, 6, 867

F. Deac, B. Iacob, E. Fischer-Fodor, G. Damian, R. Silaghi-Dumitrescu, Journal of Biochemistry, 2011, 149, 75

O. Zolog, A. Mot, F. Deac, A. Roman, E. Fischer-Fodor, R. Silaghi-Dumitrescu, Protein Journal, 2011, 30, 27

E. Antonini, M. Brunori, Hemoglobin and Myoglobin in their Reaction with Ligands; North-Holland: Amsterdam, 1971

B.J. Reeder, D.A. Svistunenko, M.A. Sharpe, M.T. Wilson, Biochemistry, 2002, 41, 367

J. Dunne, D.A. Svistunenko, A.I. Alayash, M.T. Wilson, C.E. Cooper, Advances in Experimental Medicine and Biology, 1999, 471, 9

J. Dunne, A. Caron, P. Menu, A.I. Alayash, P.W. Buehler, M.T. Wilson, R. Silaghi-Dumitrescu, B. Faivre, C.E. Cooper, Biochemical Journal, 2006, 399, 513.

STUDIA UBB CHEMIA, Volume 62 (LXII), No. 4, Tome I, June 2017

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Published

2017-12-29

How to Cite

SCURTU, F. ., POPA, A. ., & SILAGHI-DUMITRESCU, R. . (2017). PERIODATE-OXIDIZED ALGINATE AS POLYCONDENSATION REAGENT FOR HEMOGLOBIN. Studia Universitatis Babeș-Bolyai Chemia, 62(4, Tome I), 59–66. https://doi.org/10.24193/subbchem.2017.4.05