COMPARATIVE CYTOTOXICITY ASSAYS PERFORMED USING A FREE PORPHYRIN AND ITS Zn(II), Co(II) AND Cu(II) COMPLEXES. INFLUENCE OF OPTICAL AND AGGREGATION PROPERTIES

Radostina  ALEXANDROVA; Reni  KALFIN; Ramona  TUDOSE; Eugenia  FAGADAR-COSMA

doi:10.24193/subbchem.2018.4.05

Authors

Radostina ALEXANDROVA Institute of Experimental Morphology, Pathology and Anthropology, Museum of the Bulgarian Academy of Sciences, Sofia, Bulgaria. Email: rialexandrova@hotmail.com. https://orcid.org/0000-0002-7699-6479
Reni KALFIN Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria. Corresponding author: efagadar@yahoo.com. https://orcid.org/0000-0002-7764-3640
Ramona TUDOSE “Coriolan Dragulescu” Institute of Chemistry, Timisoara, Romania. Email: rtudose@acad-tim.utt.ro. https://orcid.org/0000-0001-7535-2295
Eugenia FAGADAR-COSMA “Coriolan Dragulescu” Institute of Chemistry, Timisoara, Romania. Email: efagadar@yahoo.com. https://orcid.org/0000-0001-5668-709X

DOI:

https://doi.org/10.24193/subbchem.2018.4.05

Keywords:

Zn(II), Co(II) and Cu(II)-metalloporphyrins, cytotoxicity evaluation, UV-vis spectroscopy, STEM microscopy, MTT test

Abstract

Our present approach is dealing with comparative cytotoxicity assays performed with a porphyrin base, substituted with methoxy-inductive donor substituents in the meso position, namely: 5,10,15,20-tetrakis-(p-methoxy-phenyl porphyrin (TMeOPP) and with its Zn(II), Co(II) and Cu(II) complexes (Figure 1). The cytotoxicity evaluation was performed using human (HeLa, 8 MGBA, Lep-3) and bovine (MDBK) cell lines as model systems. The influence of the compounds on cell viability and proliferation was studied by (thiazolyl blue tetrazolium bromide) MTT test and further discussed taking into consideration the main optical and aggregation properties of the free porphyrin and its different metal complexes.

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