HEMOGLOBIN-ALBUMIN CO-POLYMERS FOR BLOOD SUBSTITUTES: INCREASING THE REPRODUCIBILITY OF THE POLYMERIZATION REACTION

Florina  SCURTU; Bogdan ȚEBREAN; Mariann Kinga  ÁRKOSI; Adrian  IONELE; Radu  SILAGHI-DUMITRESCU

doi:10.24193/subbchem.2019.2.36

HEMOGLOBIN-ALBUMIN CO-POLYMERS FOR BLOOD SUBSTITUTES: INCREASING THE REPRODUCIBILITY OF THE POLYMERIZATION REACTION

Authors

Florina SCURTU Department of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Email: florinadeac@chem.ubbcluj.ro. https://orcid.org/0000-0002-2406-9163
Bogdan ȚEBREAN Department of Electrical Engineering and Measurements, Technical University, Cluj-Napoca, Romania. Email: Bogdan.Tebrean@ethm.utcluj.ro. https://orcid.org/0000-0003-4462-5670
Mariann Kinga ÁRKOSI Department of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Email: arkosi.mariann@gmail.com.
Adrian IONELE Department of Chemistry and Chemical Engineering, Babeş-Bolyai University, Cluj-Napoca, Romania. Corresponding author: radu.silaghi@ubbcluj.ro.
Radu SILAGHI-DUMITRESCU Professor PhD, Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca; Romanian Chemical Society, Romanian Society of Biochemistry and Molecular Biology, Society of Biological Inorganic Chemistry, Romania. Email: radu.silaghi@ubbcluj.ro. https://orcid.org/0000-0003-3038-7747

DOI:

https://doi.org/10.24193/subbchem.2019.2.36

Keywords:

hemoglobin, glutaraldehyde, bovine serum albumin, blood substitutes, mixer, polymerization

Abstract

Reticulation/polymerization of proteins using bifunctional reagents, with glutaraldehyde as a typical example, has a wide range of applications – of which HBOC (hemoglobin/hemerythrin based oxygen carriers) are illustrative. Here, the kinetics of the derivatization reaction of hemoglobin and albumin with glutaraldehyde are revisited, in order to establish the optimal conditions such as concentration of protein and derivatization agent for which the final product can be a reasonable candidate for blood substitutes. A purpose-built mixing device is described, which allows automated mixing of up to four reagents in less than 100 milliseconds, allowing for polymers with a more reproducible molecular weight.

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Published

2019-06-28

How to Cite

SCURTU, F. ., ȚEBREAN, B., ÁRKOSI, . M. K. ., IONELE, A. ., & SILAGHI-DUMITRESCU, R. . (2019). HEMOGLOBIN-ALBUMIN CO-POLYMERS FOR BLOOD SUBSTITUTES: INCREASING THE REPRODUCIBILITY OF THE POLYMERIZATION REACTION. Studia Universitatis Babeș-Bolyai Chemia, 64(2), 421–434. https://doi.org/10.24193/subbchem.2019.2.36