In vivo evaluation of the therapeutic effect of Streptococcus thermophilus isolated from camel milk on intestinal disorders
DOI:
https://doi.org/10.24193/subbbiol.2024.2.10Keywords:
camel milk, intestinal disorders, lactic acid bacteria, probiotics, pathogenic bacteriaAbstract
This study aimed to isolate, select, and evaluate lactic acid bacteria possessing probiotic properties. Isolates obtained from camel milk from El-Oued region in Algeria were investigated for their potential effect on intestinal disorders in Wistar rats. The results relating to the selection of probiotic strains confirm that one strain, identified as Streptococcus thermophilus exhibited the best probiotic activity, with an important tolerance to different degrees of pH and to bile salts, and a remarkable antibacterial activity and resistance to antibiotics. During in vivo studies, the administration of isolated lactic acid bacteria was evaluated after inducing intestinal disorders in rats. The microscopic observations of the histological section of the intestine showed an almost complete disappearance of the damages in the intestinal structure. The haematological parameters were in agreement with the results of the histological sections.
Article history: Received 27 April 2024; Revised 12 September 2024;
Accepted 13 October 2024; Available online 10 December 2024
References
Achemchem, F. & Abrini, J.(2005). Production de Bactériocines par des bactéries lactiques isolées à partir du jben de chèvre du nord du Maroc. Tétouan University, Maroc.
Amrouche, T.(2005). Contribution à l’étude du pouvoir immun modulateur des bifidobactéries : analyse in vitro et étude in vivo des mécanismes moléculaires impliqués: University Laval, Canada.
Azat, R., Liu, Y., Li, W., Kayir, A., Lin, D., Zhou, W., Zheng, X. (2016). Probiotic properties of lactic acid bacteria isolated from traditionally fermented Xinjiang cheese. JZUS-B, 17 (8), 597-609. https://doi.org/10.1631%2Fjzus.B1500250
Badis, A., Laouabdia, S., Guetarni, D., Kihal, M. & Ouzrout, R. (2005). Caractérisation phénotypique des bactéries lactiques isolées à partir de lait cru de chèvre de deux populations caprines locales "Arabia et kabyle". JST, 23, 30-37.
Benyoucef, A. (2019). Etude des propriétés fonctionnelles des bactéries lactiques produisant des substances antibactériennes. Ahmed Ben Bella university, Oran.
Botes, M., Van-Reenen, C.A. & Dicks, L.M.T.(2008). Evaluation of Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 as probiotics by using a gastro-intestinal model with infant milk formulation as substrate. Int. J. Food Microbiol., 128, 362-370. https://doi.org/10.1016/j.ijfoodmicro.2008.09.016
Bouguerra, A. (2021). Evaluation du potentiel probiotique des souches lactiques isolées à partir du lait de chamelle. Sétif universtity, Algeria.
Champomier-Vergès, M.C., Zagorec, M.& Fadda, S. (2010). Proteomics: a tool for understanding lactic acid bacteria adaptation to stressful environments. In: Mozzi, F., Raya, R.R., Vignolo, G.M. (eds.). Biotechnology of lactic acid bacteria: Novel applications. Blackwell publishing, pp. 57-72.
Charteris, W.P., Kelly, P.M., Morelli, L. & Collins, J.K. (1998). Antibiotic susceptibility of potentially probiotic Lactobacillus species. J. Food. Prot. 61, 1636-1643. https://doi.org/10.4315/0362-028x-61.12.1636.
Davati, N., Yazdi, F.T., Zibaee, S., Shahidi, F.& Edalatian, M.R. (2015). Study of lactic acid bacteria community from raw milk of Iranian one-humped camel and evaluation of their probiotic properties. JJM 8(5), 1-6. https://doi.org/10.5812%2Fjjm.8(5)2015.16750
Denkova, R., Goranov, B., Teneva, D., Denkova, Z. & Kostov, G. (2017). Antimicrobial activity of probiotic microorganisms: mechanisms of interaction and methods of examination. Antimicrobial. Res., Vol 2012, 201.
Guergour, H. (2011). Etude de toxicité de l’huile de Ricinus communis L sur les animaux de laboratoire". Sétif universtity, Algeria.
Guesh, M., Tesfaye, S.T., Diriba, M.& Anteneh, T.(2019). In vitro evaluation of probiotic properties of lactic acid bacteria isolated from Some traditionally fermented Ethiopian food products. Int. J. Microbiol. Vol 2019, 1-11, https://doi.org/10.1155/2019/7179514
Guiraud, J.P. & Galzy, P. (1980). L'analyse microbiologique dans les industries alimentaires. Les éditions de l'usine nouvelle; pp 239.
Kimoto-nira ,H., Kobayashi, M., Nomura, M., Sasaki,K. & Suzuki, C. (2009). Bile resistance in lactococcus lactic strains varies with cellular fatty acid composition. Int. J. Food. Microbial. 131, 183-188. https://doi.org/10.1016/j.ijfoodmicro.2009.02.021
Hadj, S. & Benchouni, O.M. (2013). Activité probiotique des hydrolysats des polysaccaride extraits de quelque plantes spontanées à caractère médical récoltées dans le Sahara algérien. Ouargla University, Algeria
Hosseini, S.V., Arlindo, S., Bohmel, K., Fernandez, C., Calo-Mata, P. & Barros-Vellazquez, J. (2009). Molecular and probiotic characterization of bacteriocin-producing Enterococcus faecium strains isolated from non-fermented animal food. J. Appl. Microbiol. 107, 1392-1403. https://doi.org/10.1111/j.1365-2672.2009.04327.x
Labioui, H., Elmoualdi, L., Benzakour, A., El Yachiou,i M., Berny, E.H., Ouhssine, M. (2009). Etude physicochimique et microbiologique de laits crus. Bull. Soc. Pharm. Bordeaux 148, 7-16.
Manjarrez-Hernandez, H.A., Gavilanes-Parra, S., Chavez-Berrocal, E., Navarro-Ocana, A. & Cravioto, A. (2000). Antigen detection in enteropathogenic Escherichia coli using secretory immunoglobulin A antibodies isolated from human breast milk. Infect. Immun. 68(9), 5030-5036. https://doi.org/10.1128/iai.68.9.5030-5036.2000
Marteau, P. & Ramb, J.C. (1998). Probiotique en gastroentérologie, base rationnel, effets démontrés et perspectives. Hepato-Gastro; Vol 5, 73-267.
Mathara, J.M., Schillinger, U., Guigas, C., Franz, C., Kutima, P.M., Mbugua, S.K., Shin H.K. & Holzapfel, W.H. (2008). Functional characteristics of Lactobacillus spp. from traditional Maasai fermented milk products in Kenya. Int. J. Food Microbial. 126, 57-64. https://doi.org/10.1016/j.ijfoodmicro.2008.04.027
Mehtar, S. & Afshar, S.A., (1983). Biotyping of Haemophilus Using API 10S, an Epidemiological Tool. J. Clin. Pathol. 36, 96-99. https://doi.org/10.1136%2Fjcp.36.1.96.
Mokoena, M.P. (2017). Lactic Acid Bacteria and Their Bacteriocins: Classification, Biosynthesis and Applications against Uropathogens. A Mini-Review. Molecules (Basel, Switzerland) 22(8), 1255. https://doi.org/10.3390/molecules22081255
Morandi, S., Cremonesi, P., Silvetti, T. & Brasca, M. (2013). Technological characterisation, antibiotic susceptibility and antimicrobial activity of wild-type Leuconostoc strains isolated from north Italian traditional cheeses. Journal of Dairy Science (JDS). 80(4), 457-466. https://doi.org/10.1017/s0022029913000447
Muller, J.A., Ross, R.P., Fitzgerald, G.F. & Stanton, C. (2009). Manufacture of probiotic bacteria. In: Charalampopoulos, D., Rastall, R. A. (eds.). Prebiotics and Probiotics Science and Technology, 725-760. https://doi.org/10.1007/978-0-387-79058-9_18
Noriega, L.M., Gueimonde, B., Sanchez, A. & Margolles, C. (2004). Effect of the adaptation to high bile salts concentrations on glycosidic activity, survival at low pH and cross-resistance to bile salts in Bifidobacterium. Int. J. Food Microbiol. 94(1), 79-86. https://doi.org/10.1016/j.ijfoodmicro.2004.01.003
Prasad, R., Sankhyan, S.K., & Karim, S.A. (1998). Growth performance of broiler rabbits fed on diets containing various types of protein supplements. Indian J. Anim. Prod. Manage. 14(4), 227-230.
Ruiz Rodríguez, L., Bleckwedel, J., Eugenia Ortiz, M., Pescuma, M. & Mozzi, F. (2017). Lactic acid bacteria, in industrial biotechnology: microorganisms, eds C. Wittmann, & J.C. Liao (Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA), 395–451. https://doi:10.1002/9783527807796.ch11.
Salhi, H. & Souaker, A. (2020). Isolation of lactic acid bacteria strains via goat and camel raw milk and evaluation of their probiotic effects: El-Oued University, Algeria.
Tabak, S. (2007). Interactions entre Helicobacter pylori responsable de maladies gastroduodénales et Bifidobacteries. Mémoire de magister, Université d’Oran.
Villeger, R. (2014). Etude in vitro des propriétés probiotiques de bactéries du genre Bacillus : Interaction avec l’hôte et effets de l’association avec un prébiotique: Limoges University, France.
Zhang, Y., Liu, X., Wang, Y., Jiang, P. & Quek, S. (2016). Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus. Food Control 59, 282-289. https://doi.org/10.1016/j.foodcont.2015.05.032.
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