DETECTION OF OCHRATOXIN A IN INSTANT COFFEE BY UPLC-MS/MS

Manuela Cristina MINCEA; Cosmin  IONAȘCU; Katalin  KIS; Vasile  OSTAFE

Authors

Manuela Cristina MINCEA Advanced Environmental Research Laboratories, “Nicholas Georgescu-Roegen” Multidisciplinary Research Platform, Timisoara, Romania. Corresponding author: vostafe@cbg.uvt.ro.
Cosmin IONAȘCU Advanced Environmental Research Laboratories, “Nicholas Georgescu-Roegen” Multidisciplinary Research Platform, Timisoara, Romania. Corresponding author: vostafe@cbg.uvt.ro.
Katalin KIS Department of Biology–Chemistry, Faculty of Chemistry, Biology and Geography, West University of Timisoara; Advanced Environmental Research Laboratories, “Nicholas Georgescu-Roegen” Multidisciplinary Research Platform, Timisoara, Romania. Corresponding author: vostafe@cbg.uvt.ro. https://orcid.org/0000-0003-4005-2932
Vasile OSTAFE Department of Biology - Chemistry Faculty of Chemistry, Biology, Geography; Advanced Environmental Research Laboratory, West University, Timisoara, Romania. Email: vasile.ostafe@e-uvt.ro. https://orcid.org/0000-0003-1352-1115

Keywords:

ochratoxin A; mycotoxin; instant coffee; contamination; UPLC-MS

Abstract

A rapid, accurate and economic procedure has been applied to extract and analyze trace level of ochratoxin A (OTA) in instant coffee. The OTA was extracted and cleaned-up by methanol extraction followed by retention on IRA-400 anion exchanger and quantified by Ultra Performance Liquid Chromatography (UPLC) with MS/MS detection. Under optimum conditions, the detection limit (S/N = 3) was 0.05 ng/g and recoveries of OTA at spiking levels of 5, 10, and 20 ng/mL, were 82.67 ± 8.05%, 96.87 ± 4.63%, and 98.25 ± 4.46% respectively.

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