FEATURES OF INFECTED VERSUS UNINFECTED CHEMICAL PROFILES RELEASED FROM HUMAN EXUDATES
DOI:
https://doi.org/10.24193/subbchem.2019.2.17Keywords:
bacterial markers, exudates, headspace profiles, diagnostic toolAbstract
Detection of bacterial volatile metabolites produced by human pathogenic bacteria is gaining continuous interest in both scientific and medical fields. Solid-phase microextraction (SPME) is a sampling technique that gained increasing attention in the last years due to its simplicity to implement and sensitivity. Volatile organic compounds (VOCs) released in the headspace over exudates infected with different bacteria were investigated in this work. GC-MS was involved for analysis. The above mentioned VOCs resulted from bacterial metabolism and the afferent processes that occur inside the biological samples. However, we identified 14 compounds emitted by the infecting pathogens, which can be assumed as bacterial markers.
References
Y. Kim, K. Park, K. Lee, Y.J. Park. Annals of Laboratory Medicine, 2015, 35, 422.
I.A. Ratiu, T. Ligor, V. Bocos-Bintintan, J. Szeliga, K. Machała, M. Jackowski, B. Buszewski, Journal of Breath Research 2019, 13, 026003, https://doi.org/10.1088/1752-7163/aaf708.
B. Buszewski, I.A. Ratiu, M. Milanowski, P. Pomastowski, T. Ligor, Journal of Breath Research, 2018, 12, 027105.
I.A. Ratiu, V. Bocoş-Bintintan, M. Turner, V. Moll, C.L.P. Thomas, Current Analitical Chemistry, 2014, 10, 488.
D. Chan, C. Leggett, K. Wang, World Journal of Gastroenterology, 2016, 2, 1639.
C.S.J. Probert, I. Ahmed, T. Khalid, E. Johnson, S. Smith, N. Ratcliffe, Journal of Gastrointestinal and Liver Diseases, 2009, 18, 337.
I.A. Ratiu, T. Ligor, V. Bocos-Bintintan, H. Al-Suod, T. Kowalkowski, K. Rafińska, B. Buszewski, Journal of Breath Research, 2017, 11, 036012.
I.A. Ratiu, T. Ligor, V. Bocos-Bintintan, B. Buszewski, Bioanalysis, 2017, 9, 1069.
M. Jünger, W. Vautz, M. Kuhns, L. Hofmann, S. Ulbricht, J.I. Baumbach, M. Quintel, T. Perl, Applied Microbiology and Biotechnology. 2012, 93:2603.
I.A. Ratiu, V. Bocos-Bintintan, A. Patrut, V. Moll, M. Turner, C.L.P. Thomas, Analitica Chimica Acta, 2017, 982, 209.
R. Huo, A. Agapiou, V. Bocos-Bintintan, L.J. Brown, C. Burns, C.S. Creaser, N.A. Devenport, B. Gao-Lau, C. Guallar-Hoyas, L. Hildebrand, A. Malkar, H.J. Martin, V.H. Moll, P. Patel, I.A. Ratiu, J.C. Reynolds, S. Sielemann, R. Slodzynski, M. Statheropoulos, M.A. Turner, W. Vautz; V.E. Wright, C.L.P. Thomas, Journal of Breath Research, 2011, 5, 046006.
V. Bocos-Bintintan, A. Smolenschi, I.A. Ratiu, Studia UBB Chemia, 2016, LXI, 203.
G.B. Ghira, I.A. Ratiu, V. Bocoş-Binţinţan, Environmental Engineering and Management Journal, 2013, 12 (2), 251.
V. Moll, V. Bocoş-Binţinţan, I.A. Ratiu, D. Ruszkiewicz, C.L.P. Thomas, Analyst, 2012, 137(6), 1458
J.L. Narayana, J. Gopal, H.F. Wu, Analyst, 2012, 137, 3372.
R.S.M. Thorn, d.m. Reynold, J. Greenman, Journal of Microbiological Methods, 2011, 84, 258.
G. Preti, E. Thaler, C.W. Hanson, M. Troy, J. Eades, A.J. Gelperin, Journal of Chromatography B, 2009, 877, 2011.
A.b. DeMilo, C.J. Lee, D.S. Moreno, A.J. Martinez, Journal of Agricultural and Food Chemistry, 1996, 44, 607.
E. Tait, J.D. Perry, S.P. Stanforth, J.R. Dean, Trends in Analitical Chemistry, 2014, 53, 117.
R.a. Quinn, K. Whiteson, Y.W. Lim, J. Zhao, D. Conrad, J.J.L. Puma, F. Rohwer, S. Widder. Biofilms Microbiomes, 2017, 2, 11.
S. Sethi, R. Nanda, T. Chakraborty, Clinical Microbiology Review, 2013, 26, 462.
S. Paczkowski, S. Schütz, Applied Microbiology & Biotechnology, 2011, 91, 917.
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