AN ANILINE HYDROGEN PHTHALATE BASED UV-VIS ASSAY FOR THE DETERMINATION OF GLUCOSE

Abstract

Rapid, easy and reliable glucose determination remains of paramount importance, be it in the context of clinical, research or industrial laboratories. Here we present a UV-vis adaptation for the determination of glucose based on a traditional paper chromatography method for glucose visualisation. Results from two independent calibration experiments (n = 3 per concentration) demonstrated a strong linear relationship between absorbance at 490 nm and glucose concentration over the tested range (0.055–2 mM) consistent with the Beer-Lambert law. High regression coefficients (R² = 0.9930, 0.9916) and comparable slopes confirmed good linearity and reproducibility. Low standard deviations and decreasing RSD values with increasing concentration indicated high precision, particularly above the lowest concentration level. The method demonstrates good sensitivity, with LOD values of 0.014–0.023 mM and LOQ values of 0.040–0.070 mM, supporting reliable quantification within the studied range. In a preliminary experiment, the assay was applied to deproteinised human plasma using the standard addition method. The estimated glucose concentration fell within the expected physiological range, indicating the potential applicability of the proposed method to complex biological samples whilst highlighting the need for further validation studies.